Tools
Tools
Telci, D., Wang, Z., Verderio, E. 
ORCID: 0000-0001-9153-8997, Humphries, M.J., Baccarini, M., Basaga, H. and Griffin, M.,
2008.
Fibronectin-tissue transglutaminase matrix rescues RGD-impaired cell adhesion through syndecan-4 and β1 integrin co-signaling.
Journal of Biological Chemistry, 283 (30), pp. 20937-20947.
|
Text
193147_1527 Verderio Publisher.pdf Download (746kB) | Preview |
Abstract
Heterotropic association of tissue transglutaminase (TG2) with extracellular matrix-associated fibronectin (FN) can restore the adhesion of fibroblasts when the integrin-mediated direct binding to FN is impaired using RGD-containing peptide. We demonstrate that the compensatory effect of the TG-FN complex in the presence of RGD-containing peptides is mediated by TG2 binding to the heparan sulfate chains of the syndecan-4 cell surface receptor. This binding mediates activation of protein kinase Cα (PKCα) and its subsequent interaction with β1 integrin since disruption of PKCα binding to β1 integrins with a cell-permeant competitive peptide inhibits cell adhesion and the associated actin stress fiber formation. Cell signaling by this process leads to the activation of focal adhesion kinase and ERK1/2 mitogen-activated protein kinases. Fibroblasts deficient in Raf-1 do not respond fully to the TG-FN complex unless either the full-length kinase competent Raf-1 or the kinase-inactive domain of Raf-1 is reintroduced, indicating the involvement of the Raf-1 protein in the signaling mechanism. We propose a model for a novel RGD-independent cell adhesion process that could be important during tissue injury and/or remodeling whereby TG-FN binding to syndecan-4 activates PKCα leading to its association with β1 integrin, reinforcement of actin-stress fiber organization, and MAPK pathway activation.
| Item Type: | Journal article |
|---|---|
| Publication Title: | Journal of Biological Chemistry |
| Creators: | Telci, D., Wang, Z., Verderio, E., Humphries, M.J., Baccarini, M., Basaga, H. and Griffin, M. |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date: | 2008 |
| Volume: | 283 |
| Number: | 30 |
| Rights: | Copyright © 2008 by American Society for Biochemistry and Molecular Biology. |
| Divisions: | Schools > School of Science and Technology |
| Record created by: | EPrints Services |
| Date Added: | 09 Oct 2015 10:15 |
| Last Modified: | 09 Jun 2017 13:23 |
| URI: | https://irep.ntu.ac.uk/id/eprint/10065 |
Actions (login required)
 |
Edit View |
Views
Views per month over past year
Downloads
Downloads per month over past year
