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Kapoor, K.N., Barry, D.T., Rees, R.C. 
ORCID: 0000-0002-4574-4746, Dodi, I.A., McArdle, S.E.B. ORCID: 0000-0001-6929-9782, Creaser, C.S. and Bonner, P.L.R. ORCID: 0000-0001-9015-3403,
2009.
Estimation of peptide concentration by a modified bicinchoninic acid assay.
Analytical Biochemistry, 393 (1), pp. 138-140.
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Abstract
Although biuret based protein assays are theoretically applicable to peptide measurement, there is a high level of inter-peptide variation, determined largely by peptide hydrophobicity. This variation in peptide reactivity can be significantly reduced by heat-denaturation of peptides at 95 °C for 5 minutes in the presence of 0.1 M NaOH containing 1% (w/v) SDS, prior to incubation for 30 min at 37 °C in BCA standard working reagent. This modification to the standard bicinchoninic acid (BCA) assay protocol allows for an accurate, rapid and economical estimation of the peptide concentration within an unknown sample.
| Item Type: | Journal article | ||||
|---|---|---|---|---|---|
| Publication Title: | Analytical Biochemistry | ||||
| Creators: | Kapoor, K.N., Barry, D.T., Rees, R.C., Dodi, I.A., McArdle, S.E.B., Creaser, C.S. and Bonner, P.L.R. | ||||
| Publisher: | Elsevier | ||||
| Date: | 2009 | ||||
| Volume: | 393 | ||||
| Number: | 1 | ||||
| Identifiers: |
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| Divisions: | Schools > School of Science and Technology | ||||
| Record created by: | EPrints Services | ||||
| Date Added: | 09 Oct 2015 10:25 | ||||
| Last Modified: | 24 Nov 2021 13:56 | ||||
| URI: | https://irep.ntu.ac.uk/id/eprint/12618 |
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