Estimation of peptide concentration by a modified bicinchoninic acid assay

Kapoor, KN, Barry, DT, Rees, RC ORCID: https://orcid.org/0000-0002-4574-4746, Dodi, IA, McArdle, SEB ORCID: https://orcid.org/0000-0001-6929-9782, Creaser, CS and Bonner, PLR ORCID: https://orcid.org/0000-0001-9015-3403, 2009. Estimation of peptide concentration by a modified bicinchoninic acid assay. Analytical Biochemistry, 393 (1), pp. 138-140.

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Abstract

Although biuret based protein assays are theoretically applicable to peptide measurement, there is a high level of inter-peptide variation, determined largely by peptide hydrophobicity. This variation in peptide reactivity can be significantly reduced by heat-denaturation of peptides at 95 °C for 5 minutes in the presence of 0.1 M NaOH containing 1% (w/v) SDS, prior to incubation for 30 min at 37 °C in BCA standard working reagent. This modification to the standard bicinchoninic acid (BCA) assay protocol allows for an accurate, rapid and economical estimation of the peptide concentration within an unknown sample.

Item Type:	Journal article
Publication Title:	Analytical Biochemistry
Creators:	Kapoor, K.N., Barry, D.T., Rees, R.C., Dodi, I.A., McArdle, S.E.B., Creaser, C.S. and Bonner, P.L.R.
Publisher:	Elsevier
Date:	2009
Volume:	393
Number:	1
Identifiers:	Number Type 10.1016/j.ab.2009.06.016 DOI
Divisions:	Schools > School of Science and Technology
Record created by:	EPrints Services
Date Added:	09 Oct 2015 10:25
Last Modified:	24 Nov 2021 13:56
URI:	https://irep.ntu.ac.uk/id/eprint/12618

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