Reduced tubulin tyrosination as an early marker of mercury toxicity in differentiating N2a cells

LAWTON, M., IQBAL, M., KONTOVRAKI, M., LLOYD MILLS, C. and HARGREAVES, A.J., 2007. Reduced tubulin tyrosination as an early marker of mercury toxicity in differentiating N2a cells. Toxicology in Vitro, 21 (7), pp. 1258-1261. ISSN 0887-2333

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Abstract

The aims of this work were to compare the effects of methyl mercury chloride and Thimerosal on neurite/process outgrowth and microtubule proteins in differentiating mouse N2a neuroblastoma and rat C6 glioma cells. Exposure for 4 h to sublethal concentrations of both compounds inhibited neurite outgrowth to a similar extent in both cells lines compared to controls. In the case of N2a cells, this inhibitory effect by both compounds was associated with a fall in the reactivity of western blots of cell extracts with monoclonal antibody T1A2, which recognises C-terminally tyrosinated α-tubulin. By contrast, reactivity with monoclonal antibody B512 (which recognises total α-tubulin) was unaffected at the same time point. These findings suggest that decreased tubulin tyrosination represents a neuron-specific early marker of mercury toxicity associated with impaired neurite outgrowth.

Item Type: Journal article
Publication Title: Toxicology in Vitro
Creators: Lawton, M., Iqbal, M., Kontovraki, M., Lloyd Mills, C. and Hargreaves, A.J.
Publisher: Elsevier
Date: 2007
Volume: 21
Number: 7
ISSN: 0887-2333
Identifiers:
NumberType
10.1016/j.tiv.2007.03.018DOI
Rights: Copyright © 2007 Elsevier Ltd All rights reserved.
Divisions: Schools > School of Science and Technology
Depositing User: EPrints Services
Date Added: 09 Oct 2015 10:33
Last Modified: 23 Aug 2016 09:10
URI: http://irep.ntu.ac.uk/id/eprint/14732

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