Reduced tubulin tyrosination as an early marker of mercury toxicity in differentiating N2a cells

Lawton, M, Iqbal, M, Kontovraki, M, Lloyd Mills, C and Hargreaves, AJ ORCID: 0000-0001-9754-5477, 2007. Reduced tubulin tyrosination as an early marker of mercury toxicity in differentiating N2a cells. Toxicology in Vitro, 21 (7), pp. 1258-1261. ISSN 0887-2333

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The aims of this work were to compare the effects of methyl mercury chloride and Thimerosal on neurite/process outgrowth and microtubule proteins in differentiating mouse N2a neuroblastoma and rat C6 glioma cells. Exposure for 4 h to sublethal concentrations of both compounds inhibited neurite outgrowth to a similar extent in both cells lines compared to controls. In the case of N2a cells, this inhibitory effect by both compounds was associated with a fall in the reactivity of western blots of cell extracts with monoclonal antibody T1A2, which recognises C-terminally tyrosinated α-tubulin. By contrast, reactivity with monoclonal antibody B512 (which recognises total α-tubulin) was unaffected at the same time point. These findings suggest that decreased tubulin tyrosination represents a neuron-specific early marker of mercury toxicity associated with impaired neurite outgrowth.

Item Type: Journal article
Publication Title: Toxicology in Vitro
Creators: Lawton, M., Iqbal, M., Kontovraki, M., Lloyd Mills, C. and Hargreaves, A.J.
Publisher: Elsevier
Date: 2007
Volume: 21
Number: 7
ISSN: 0887-2333
Rights: Copyright © 2007 Elsevier Ltd All rights reserved.
Divisions: Schools > School of Science and Technology
Depositing User: EPrints Services
Date Added: 09 Oct 2015 10:33
Last Modified: 09 Jun 2017 13:33

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