Phenotypic microarrays suggest Escherichia coli ST131 is not a metabolically distinct lineage of extra-intestinal pathogenic E. coli

Alqasim, A., Emes, R. ORCID: 0000-0001-6855-5481, Clark, G., Newcombe, J., La Ragione, R. and McNally, A. ORCID: 0000-0002-3099-630X, 2014. Phenotypic microarrays suggest Escherichia coli ST131 is not a metabolically distinct lineage of extra-intestinal pathogenic E. coli. PLoS ONE, 9 (2). ISSN 1932-6203

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Abstract

Extraintestinal pathogenic E. coli (ExPEC) are the major aetiological agent of urinary tract infections (UTIs) in humans. The emergence of the CTX-M producing clone E. coli ST131 represents a major challenge to public health worldwide. A recent study on the metabolic potential of E. coli isolates demonstrated an association between the E. coli ST131 clone and enhanced utilisation of a panel of metabolic substrates. The studies presented here investigated the metabolic potential of ST131 and other major ExPEC ST isolates using 120 API test reagents and found that ST131 isolates demonstrated a lower metabolic activity for 5 of 120 biochemical tests in comparison to non-ST131 ExPEC isolates. Furthermore, comparative phenotypic microarray analysis showed a lack of specific metabolic profile for ST131 isolates countering the suggestion that these bacteria are metabolically fitter and therefore more successful human pathogens.

Item Type:	Journal article
Publication Title:	PLoS ONE
Creators:	Alqasim, A., Emes, R., Clark, G., Newcombe, J., La Ragione, R. and McNally, A.
Publisher:	PLoS (Public Library of Science)
Place of Publication:	San Francisco, CA, USA
Date:	2014
Volume:	9
Number:	2
ISSN:	1932-6203
Identifiers:	Number Type 10.1371/journal.pone.0088374 DOI
Rights:	© 2014 Alqasim et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Divisions:	Schools > School of Science and Technology
Record created by:	EPrints Services
Date Added:	09 Oct 2015 10:35
Last Modified:	08 Feb 2023 12:02
URI:	https://irep.ntu.ac.uk/id/eprint/15260

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