Investigation of a model FRET system with multiple donors and acceptors. Allowing validation of data interpretation for studies of cells

Gumbs, WGA, 2011. Investigation of a model FRET system with multiple donors and acceptors. Allowing validation of data interpretation for studies of cells. MPhil, Nottingham Trent University.

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Abstract

In this study the foundations are laid for using a fluorescence microscopy system to view multiple fluorescent species in mammalian cells as a possible technique in investigating the oligomers status of GPCRs. The system utilises spectrally resolved intensity, anisotropy and lifetime measurements. All these techniques are proven to be useable and comparable to the literature. Its weaknesses such as illumination intensity are recognised and its more novel capabilities are utilised. Cho cells are imaged using a confocal microscope and the plasmid sequences providing them with fluorescence through YFP and CFP are established to be correct. In the pursuit of fine tuning the microscope system to allow the live cells images to be taken and any FRET recognised, a model system from the literature is examined. However this turns out to be a fascinating system all of its own; the calculations lead to conclusions that the FRET occurring are in fact predicting inner filter effects along with other unidentified processes. This study looks at why this is the case.

Item Type: Thesis
Creators: Gumbs, W.G.A.
Date: 2011
Rights: This work is the intellectual property of the author (Note: if there are other owners of the IP, as a consequence of any statement issued under paragraph 12 of Section 14A, they must also be named here). You may copy up to 5% of this work for private study, or personal, non-commercial research. Any re-use of the information contained within this document should be fully referenced, quoting the author, title, university, degree level and pagination. Queries or requests for any other use, or if a more substantial copy is required, should be directed in the owner(s) of the Intellectual Property Rights.
Divisions: Schools > School of Science and Technology
Depositing User: EPrints Services
Date Added: 09 Oct 2015 09:34
Last Modified: 09 Oct 2015 09:34
URI: http://irep.ntu.ac.uk/id/eprint/153

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