Identification of hyperinvasive Campylobacter jejuni strains isolated from poultry and human clinical sources

Fearnley, C, Manning, G, Bagnall, MC, Afzal Javed, M, Wassenaar, TM and Newell, DG, 2008. Identification of hyperinvasive Campylobacter jejuni strains isolated from poultry and human clinical sources. Journal of Medical Microbiology, 57 (5), pp. 570-580. ISSN 1473-5644

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Abstract

Campylobacter jejuni causes gastroenteritis with a variety of symptoms in humans. In the absence of a suitable animal model, in vitro models have been used to study virulence traits such as invasion and toxin production. In this study, 113 C. jejuni isolates from poultry and poultry-related (n=74) environments as well as isolates from human cases (n=39) of campylobacteriosis and bacteraemia were tested for invasiveness using INT 407 cells. The method was sufficiently reproducible to observe a spectrum of invasiveness amongst strains. As a result, strains were classified as low, high and hyper-invasive. The majority of strains (poultry and human) were low invaders (82 % and 88 %, respectively). High invasion was found for 5 % of human strains and 11 % of poultry-related isolates. However, only 1 % of poultry strains were classified as hyperinvasive compared to 13 % of human isolates (P=0.0182). Of those isolates derived from the blood of bacteraemic patients, 20 % were hyperinvasive, though this correlation was not statistically significant. An attempt was made to correlate invasiveness with the presence of seven genes previously reported to be associated with virulence. Most of these genes did not correlate with invasiveness, but gene cj0486 was weakly over-represented, and a negative correlation was observed for the gene ciaB. This trend was stronger when the two genes were analysed together, thus ciaB– cj0486+ was over-represented in high and hyperinvasive strains, with low invaders more commonly found to lack these genes (P=0.0064).

Item Type: Journal article
Alternative Title: The identification of hyperinvasive Campylobacter jejuni strains in poultry and human clinical isolates
Hyperinvasive Campylobacter jejuni strains [running title]
Publication Title: Journal of Medical Microbiology
Creators: Fearnley, C., Manning, G., Bagnall, M.C., Afzal Javed, M., Wassenaar, T.M. and Newell, D.G.
Publisher: Society for General Microbiology
Date: 2008
Volume: 57
Number: 5
ISSN: 1473-5644
Identifiers:
NumberType
10.1099/jmm.0.47803-0DOI
Rights: This is an author manuscript that has been accepted for publication in Journal of Microbiology, copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in Journal of Microbiology. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of ‘Fair Use of Copyrighted Materials’ (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at http://intl-jmm.sgmjournals.org/, and is freely available without a subscription.
© 2008 Society for General Microbiology
Divisions: Schools > School of Science and Technology
Depositing User: EPrints Services
Date Added: 09 Oct 2015 10:39
Last Modified: 23 Aug 2016 09:11
URI: http://irep.ntu.ac.uk/id/eprint/16101

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