Development of an hydrophobic fluoro-silica surface for studying homotypic cancer cell aggregation–disaggregation as a single dynamic process in vitro

NICKLIN, M., REES, R.C., POCKLEY, A.G. and PERRY, C.C., 2014. Development of an hydrophobic fluoro-silica surface for studying homotypic cancer cell aggregation–disaggregation as a single dynamic process in vitro. Biomaterials Science, 2 (10), pp. 1486-1496. ISSN 2047-4830

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Abstract

Under normal conditions the detachment of anchorage-dependant cells from their extracellular matrix typically induces programmed cell death which is mediated through a pathway referred to as anoikis. However, a resistance to anoikis in cancer enables the migration of cells from the primary tumour and the establishment of aggressive metastatic disease. Although cancer cell aggregation is known to be an important mechanism within anoikis resistance, research into the underlying mechanisms that govern this process remain problematic as commercially available tissue culture material can only sustain 2D monolayer or 3D aggregate/spheroid cultures. This necessitates the development of a system that can accommodate for cancer cell aggregation–disaggregation as a single dynamic process, without the disruption of passaging cells between alternate substrates. This study describes a procedure for modifying tissue culture polystyrene (TCP) to produce a fluoro-silica (FS) surface which preferentially promotes the deposition of a distinct profile of proteins/factors from serum which mediate the transient aggregation of human breast cancer cell lines. This modified surface therefore provides an experimental platform for better understanding cancer cell aggregation–disaggregation events in vitro, and their influence on the establishment of metastatic disease in patients with cancer.

Item Type: Journal article
Publication Title: Biomaterials Science
Creators: Nicklin, M., Rees, R.C., Pockley, A.G. and Perry, C.C.
Publisher: Royal Society of Chemistry
Place of Publication: Cambridge
Date: 2014
Volume: 2
Number: 10
ISSN: 2047-4830
Identifiers:
NumberType
10.1039/C4BM00194JDOI
Divisions: Schools > School of Science and Technology
Depositing User: EPrints Services
Date Added: 09 Oct 2015 10:44
Last Modified: 09 Jun 2017 13:37
URI: http://irep.ntu.ac.uk/id/eprint/17508

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