Nitric oxide and nitrous oxide production and cycling during dissimulatory nitrite reduction by Pseudomonas perfectomarina

Zafiriou, OC, Hanley, QS ORCID: 0000-0002-8189-9550 and Snyder, G, 1989. Nitric oxide and nitrous oxide production and cycling during dissimulatory nitrite reduction by Pseudomonas perfectomarina. Journal of Biological Chemistry, 264 (10), pp. 5694-5699.

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Abstract

The denitrifier Pseudomonas perfectomarina reduced nitrite under conditions of kinetic competition between cells and gas sparging for extracellular dissolved nitric and nitrous oxides, NOaq and N2Oaq, in a chemically defined marine medium. Time courses of nitrite reduction and NOg and N2Og removal were integrated to give NOg , and N2Og yields. At high sparging rates, the NOg yield was >50% of nitrite-N reduced, and the yield of NOg + N2Og was ~75%. Hence interrupted denitrification yields NOaq and N2Oaq as major products. The yields varied with sparging rates in agreement with a quantitative model of denitrification (Betlach, M. P., and Tiedje, J. M. (1981) Appl. Environ. Microbiol. 42, 1074-1084) that applies simplified Michaelis-Menten kinetics to NO2 > NOaq > N2Oaq > N2. The fit gave an estimate of the maximum scavengeable NOaq yield of 73 ± 8% of nitrite-N. Thus a minor path independent of NOaq is also required. The fit of the model to data at lower sparging rates, where normal denitrification products predominate, implies that the extracellular NOaq pool yield is independent of gas sparging rate. Thus in P. perfectomarina NOaq and N2Oaq are intermediates, or facilely equilibrate with true intermediates, during complete denitrification. The recovery of most nitrite-N as NO and/or N20 under perturbed conditions is not an artifact of irreversible product removal, but an attribute of denitrification in this species, and most probably it is characteristic of denitrification in other species as well.

Item Type: Journal article
Publication Title: Journal of Biological Chemistry
Creators: Zafiriou, O.C., Hanley, Q.S. and Snyder, G.
Publisher: American Society for Biochemistry and Molecular Biology
Date: 1989
Volume: 264
Number: 10
Rights: © 1989 The American Society for Biochemistry and Molecular Biology, Inc.
Divisions: Schools > School of Science and Technology
Depositing User: EPrints Services
Date Added: 09 Oct 2015 10:56
Last Modified: 09 Jun 2017 13:45
URI: http://irep.ntu.ac.uk/id/eprint/20276

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