A multiphase model for tissue construct growth in a perfusion bioreactor

O'Dea, R.D., Waters, S.L. and Byrne, H.M., 2010. A multiphase model for tissue construct growth in a perfusion bioreactor. Mathematical Medicine and Biology, 27 (2), pp. 95-127.

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Abstract

The growth of a cell population within a rigid porous scaffold in a perfusion bioreactor is studied, using a three phase continuum model of the type presented by Lemon et al. (2006) to represent the cell population (and attendant extracellular matrix), culture medium and porous scaffold. The bioreactor system is modelled as a two-dimensional channel containing the cell-seeded rigid porous scaffold (tissue construct) which is perfused with culture medium. The study concentrates on (i) cell-cell and cell-scaffold interactions and, (ii) the impact of mechanotransduction mechanisms on construct composition. A numerical and analytical analysis of the model equations is presented and, depending upon the relative importance of cell aggregation and repulsion, markedly different cell movement is revealed. Additionally, mechanotransduction effects due to cell density, pressure and shear stress-mediated tissue growth are shown to generate qualitative differences in the composition of the resulting construct. The results of our simulations indicate that this model formulation (in conjunction with appropriate experimental data) has the potential to provide a means of identifying the dominant regulatory stimuli in a cell population.

Item Type: Journal article
Publication Title: Mathematical Medicine and Biology
Creators: O'Dea, R.D., Waters, S.L. and Byrne, H.M.
Publisher: Oxford University Press
Date: 2010
Volume: 27
Number: 2
Identifiers:
NumberType
10.1093/imammb/dqp003DOI
Rights: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Mathematical Medicine and Biology following peer review. The definitive publisher-authenticated version O'DEA, R.D., WATERS, S.L. and BYRNE, H.M., 2010. A multiphase model for tissue construct growth in a perfusion bioreactor. Mathematical Medicine and Biology, 27 (2), pp. 95-127 is available online at: http://dx.doi.org/10.1093/imammb/dqp003.
Divisions: Schools > School of Science and Technology
Depositing User: EPrints Services
Date Added: 09 Oct 2015 11:00
Last Modified: 23 Aug 2016 09:13
URI: http://irep.ntu.ac.uk/id/eprint/21377

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