Transposon mutagenesis in a hyper-invasive clinical isolate of Campylobacter jejuni reveals a number of genes with potential roles in invasion

Javed, M.A., Grant, A.J., Bagnall, M.C., Maskell, D.J., Newell, D.G. and Manning, G., 2010. Transposon mutagenesis in a hyper-invasive clinical isolate of Campylobacter jejuni reveals a number of genes with potential roles in invasion. Microbiology, 156 (4), pp. 1134-1143. ISSN 1350-0872

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Abstract

Transposon mutagenesis has been applied to a hyper-invasive clinical isolate of Campylobacter jejuni, 01/51. A random transposon mutant library was screened in an in vitro assay of invasion and 26 mutants with a significant reduction in invasion were identified. Given that the invasion potential of C. jejuni is relatively poor compared to other enteric pathogens, the use of a hyper-invasive strain was advantageous as it greatly facilitated the identification of mutants with reduced invasion. The location of the transposon insertion in 23 of these mutants has been determined; all but three of the insertions are in genes also present in the genome-sequenced strain NCTC 11168. Eight of the mutants contain transposon insertions in one region of the genome (∼14 kb), which when compared with the genome of NCTC 11168 overlaps with one of the previously reported plasticity regions and is likely to be involved in genomic variation between strains. Further characterization of one of the mutants within this region has identified a gene that might be involved in adhesion to host cells.

Item Type: Journal article
Alternative Title: Campylobacter jejuni mutants with reduced invasion [running title]
Description: This is an author manuscript that has been accepted for publication in Microbiology, copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in Microbiology. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of 'Fair Use of Copyrighted Materials' (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at http://mic.sgmjournals.org, and is freely available without a subscription.
Publication Title: Microbiology
Creators: Javed, M.A., Grant, A.J., Bagnall, M.C., Maskell, D.J., Newell, D.G. and Manning, G.
Publisher: Society for General Microbiology
Date: 2010
Volume: 156
Number: 4
ISSN: 1350-0872
Identifiers:
NumberType
10.1099/mic.0.033399-0DOI
Rights: Copyright © 2010 Society for General Microbiology
Divisions: Schools > School of Science and Technology
Depositing User: EPrints Services
Date Added: 09 Oct 2015 11:06
Last Modified: 23 Aug 2016 09:13
URI: http://irep.ntu.ac.uk/id/eprint/22874

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