Tools
Tools
Di, S., Del, D., Verderio, E. 
ORCID: 0000-0001-9153-8997, Hargreaves, A.J., Scarpellini, A., Cai, G., Cresti, M., Faleri, C., Iorio, R.A., Hirose, S., Furutani, Y., Coutts, I.G., Griffin, M., Bonner, P.L. ORCID: 0000-0001-9015-3403 and Serafini-Fracassini, D.,
2010.
An extracellular transglutaminase is required for apple pollen tube growth.
Biochemical Journal, 429, pp. 261-271.
|
Text
200642_7049 Verderio-Edwards Postprint Edited.pdf Download (1MB) | Preview |
Abstract
An extracellular form of the calcium-dependent protein-crosslinking enzyme TGase (transglutaminase) was demonstrated to be involved in the apical growth of Malus domestica pollen tube. Apple pollen TGase and its substrates were co-localized within aggregates on the pollen tube surface, as determined by indirect immunofluorescence staining and the in situ cross-linking of fluorescently labelled substrates. TGase-specific inhibitors and an anti-TGase monoclonal antibody blocked pollen tube growth, whereas incorporation of a recombinant fluorescent mammalian TGase substrate (histidine-tagged green fluorescent protein:His6– Xpr–GFP) into the growing tube wall enhanced tube length and germination, consistent with a role of TGase as a modulator of cell wall building and strengthening. The secreted pollen TGase catalysed the cross-linking of both PAs (polyamines) into proteins (released by the pollen tube) and His6-Xpr-GFP into endogenous or exogenously added substrates. A similar distribution of TGase activitywas observed in planta on pollen tubes germinating inside the style, consistent with a possible additional role for TGase in the interaction between the pollen tube and the style during fertilization.
| Item Type: | Journal article | ||||
|---|---|---|---|---|---|
| Publication Title: | Biochemical Journal | ||||
| Creators: | Di, S., Del, D., Verderio, E., Hargreaves, A.J., Scarpellini, A., Cai, G., Cresti, M., Faleri, C., Iorio, R.A., Hirose, S., Furutani, Y., Coutts, I.G., Griffin, M., Bonner, P.L. and Serafini-Fracassini, D. | ||||
| Publisher: | Portland Press | ||||
| Date: | 2010 | ||||
| Volume: | 429 | ||||
| Identifiers: |
|
||||
| Rights: | © 2010 Portland Press Ltd. The final version of record is available at http://www.biochemj.org/bj/default.htm. | ||||
| Divisions: | Schools > School of Science and Technology | ||||
| Record created by: | EPrints Services | ||||
| Date Added: | 09 Oct 2015 11:09 | ||||
| Last Modified: | 09 Jun 2017 13:51 | ||||
| URI: | https://irep.ntu.ac.uk/id/eprint/23687 |
Actions (login required)
 |
Edit View |
Views
Views per month over past year
Downloads
Downloads per month over past year
