An extracellular transglutaminase is required for apple pollen tube growth

Di, S., Del, D., Verderio, E. ORCID: 0000-0001-9153-8997, Hargreaves, A.J., Scarpellini, A., Cai, G., Cresti, M., Faleri, C., Iorio, R.A., Hirose, S., Furutani, Y., Coutts, I.G., Griffin, M., Bonner, P.L. ORCID: 0000-0001-9015-3403 and Serafini-Fracassini, D., 2010. An extracellular transglutaminase is required for apple pollen tube growth. Biochemical Journal, 429, pp. 261-271.

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Abstract

An extracellular form of the calcium-dependent protein-crosslinking enzyme TGase (transglutaminase) was demonstrated to be involved in the apical growth of Malus domestica pollen tube. Apple pollen TGase and its substrates were co-localized within aggregates on the pollen tube surface, as determined by indirect immunofluorescence staining and the in situ cross-linking of fluorescently labelled substrates. TGase-specific inhibitors and an anti-TGase monoclonal antibody blocked pollen tube growth, whereas incorporation of a recombinant fluorescent mammalian TGase substrate (histidine-tagged green fluorescent protein:His6– Xpr–GFP) into the growing tube wall enhanced tube length and germination, consistent with a role of TGase as a modulator of cell wall building and strengthening. The secreted pollen TGase catalysed the cross-linking of both PAs (polyamines) into proteins (released by the pollen tube) and His6-Xpr-GFP into endogenous or exogenously added substrates. A similar distribution of TGase activitywas observed in planta on pollen tubes germinating inside the style, consistent with a possible additional role for TGase in the interaction between the pollen tube and the style during fertilization.

Item Type: Journal article
Publication Title: Biochemical Journal
Creators: Di, S., Del, D., Verderio, E., Hargreaves, A.J., Scarpellini, A., Cai, G., Cresti, M., Faleri, C., Iorio, R.A., Hirose, S., Furutani, Y., Coutts, I.G., Griffin, M., Bonner, P.L. and Serafini-Fracassini, D.
Publisher: Portland Press
Date: 2010
Volume: 429
Identifiers:
NumberType
10.1042/BJ20100291DOI
Rights: © 2010 Portland Press Ltd. The final version of record is available at http://www.biochemj.org/bj/default.htm.
Divisions: Schools > School of Science and Technology
Depositing User: EPrints Services
Date Added: 09 Oct 2015 11:09
Last Modified: 09 Jun 2017 13:51
URI: http://irep.ntu.ac.uk/id/eprint/23687

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