HORTON, R., 2006. Identification of candidate tumour antigen peptides with immunogenic potential for use in cancer immunotherapy. PhD, Nottingham Trent University.
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It is now understood that CD4+ T cells play a central role in antitumour immunity; they are important in the priming of CTL and can augment cytotoxicity; they are also responsible for enhancing antitumour responses via indirect mechanisms such as recruitment of accessory cells to the tumour site and they are crucial in the formation of memory T cells. CD4+ T cells recognise antigen in the context of peptide presented on MHC class II molecules, therefore recent research has focused on the identification of these peptides in order to formulate more effective immunotherapeutic strategies. As such, the aim of this study was to identify novel MHC class II HLA-DR1 and HLA-DR4 restricted immunogenic peptides derived from the MART-1 and Tyrosinase tumour antigens. A computer algorithm (SYFPEITHI; available on the World Wide Web) was used to predict immunogenic peptides from the two tumour antigens; these peptides were then used to immunise HLA-DR1 and HLA-DR4 transgenic mice in order to assess their immunogenicity. At the same time efforts were made to optimise the screening process by fully characterising the BM-DC used in proliferation assays and employing antioxidants in conjunction with T cell culture.
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|Divisions:||Schools > School of Science and Technology|
|Depositing User:||EPrints Services|
|Date Added:||09 Oct 2015 09:35|
|Last Modified:||09 Oct 2015 09:35|
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