Increase in bacteraemia cases in the East Midlands region of the UK due to MDREscherichia coliST73: high levels of genomic and plasmid diversity in causative isolates

Alhashash, F., Wang, X., Paszkiewicz, K., Diggle, M., Zong, Z. and McNally, A. ORCID: 0000-0002-3099-630X, 2016. Increase in bacteraemia cases in the East Midlands region of the UK due to MDREscherichia coliST73: high levels of genomic and plasmid diversity in causative isolates. Journal of Antimicrobial Chemotherapy, 71 (2), pp. 339-343. ISSN 0305-7453

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Abstract

Objectives: To determine the population structure of E. coli ST73 isolated from human bacteraemia and urinary tract infections.
Methods: The genomes of 22 E.coli ST73 isolates were sequenced using the Illumina HiSeq platform. High resolution SNP typing was used to create a phylogenetic tree. Comparative genomics were also performed using a pangenome approach. In silico and S1-PFGE plasmid profiling was conducted, and isolates were checked for their ability to survive exposure to human serum.
Results: E.coli ST73 isolates circulating in clinically unrelated episodes show a high degree of diversity at a whole genome level, though exhibit conservation in gene content, particularly in virulence associated gene carriage. The isolates also contain a highly diverse plasmid pool that confers multi-drug resistance via carriage of CTX-M genes. All strains are highly serum resistant and uniformly carry genes shown to be essential for serum resistance.
Conclusions: Our data shows that a rise in incidence of multi-drug resistant E.coli ST73 clinical isolates is not due to a circulating outbreak strain as in E.coli ST131. Rather the ST73 circulating strains are distantly related and carry a diverse set of resistance plasmids. This suggests that the evolutionary events behind emergence of drug resistant E.coli differ between lineages.

Item Type: Journal article
Alternative Title: E.coli ST73 genome analysis [running title]
Publication Title: Journal of Antimicrobial Chemotherapy
Creators: Alhashash, F., Wang, X., Paszkiewicz, K., Diggle, M., Zong, Z. and McNally, A.
Publisher: Oxford University Press
Date: 2016
Volume: 71
Number: 2
ISSN: 0305-7453
Identifiers:
NumberType
10.1093/jac/dkv365DOI
Divisions: Schools > School of Science and Technology
Depositing User: Jonathan Gallacher
Date Added: 17 Nov 2015 14:08
Last Modified: 09 Jun 2017 13:57
URI: http://irep.ntu.ac.uk/id/eprint/26384

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