Comparative pathogenesis of Yersinia enterocolitica biotypes

Alenezi, D., 2015. Comparative pathogenesis of Yersinia enterocolitica biotypes. PhD, Nottingham Trent University.

Preview

Text Alenezi_2015.pdf - Published version Download (2MB) | Preview

Abstract

Yersinia enterocolitica is a well known food-borne bacterium which belongs to the Enterobacteriaceae family. Y. enterocolitica is pathogenic for humans and it causes a wide spectrum of diseases ranging from self-limiting gastrointestinal diseases to fatal sepsis depending on the age and the immunity of the infected person. Oral ingestion of undercooked raw meat products, unpasteurised milk and contaminated water are the main sources of acquisition of this bacterium. Y. enterocolitica strains are categorized into six biotypes (1A, 1B, 2, 3, 4 &5). These six biotypes are further classified into high pathogenic, low pathogenic and non-pathogenic biotypes depending on their pathogenicity in the mouse infection model. This project compared the pathogenic potential of Yersinia enterocolitica high pathogenic, low pathogenic and non-pathogenic biotypes. The HEp-2 laryngitic human epithelial cell line and the alternative infection model Galleria mellonella greater wax moth larvae are the main infection models used to investigate the pathogenic potential of Y. enterocolitica biotypes. A diverse collection of Y. enterocolitica strains was used by which this strains collection is encompassing all six biotypes and all major serotypes. These strains were isolated from human and animal origins. Here novel results are presented showing new infection phenotypes of the Y. enterocolitica strains. All Y. enterocolitica biotypes strains were invasive to the HEp-2 epithelial cell line and pathogenic to the Galleria insect model. The non-pathogenic biotype was the most lethal in the Galleria model while the high pathogenic biotype shows little to no pathogenicity. The low pathogenic biotypes were moderately pathogenic to the Galleria insect model. The pYV plasmid showed a minor role in modulating the virulence in the Y. enterocolitica pathogenic biotypes. Investigating the in vivo Y. enterocolitica-Galleria interaction showed that Y. enterocolitica pathogenic biotypes bacterial cells were growing inside the Galleria larvae while the non-pathogenic biotype bacterial cells were dying. The heat-killed Y. enterocolitica strains were completely avirulent to the Galleria larvae.

Item Type:	Thesis
Creators:	Alenezi, D.
Date:	2015
Rights:	This work is the intellectual property of the author. You may copy up to 5% of this work for private study, or personal, non-commercial research. Any re-use of the information contained within this document should be fully referenced, quoting the author, title, university , degree level and pagination. Queries or requests for any other use, or if a more substantial copy is required, should be directed to the owner(s) of the Intellectual Property Rights.
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	12 Feb 2016 09:33
Last Modified:	19 Jul 2016 15:00
URI:	https://irep.ntu.ac.uk/id/eprint/26943

Actions (login required)

Edit View

Views

Downloads