Tools
Tools
Almajed, F.S. and Forsythe, S.J. 
ORCID: 0000-0002-6709-0712,
2016.
Cronobacter sakazakii clinical isolates overcome host barriers and evade the immune response.
Microbial Pathogenesis, 90, pp. 55-63.
ISSN 0882-4010
|
Text
PubSub4408_Frosythe.pdf - Post-print Download (751kB) | Preview |
Abstract
Cronobacter sakazakii is the most frequently clinically isolated species of the Cronobacter genus. However the virulence factors of C. sakazakii including their ability to overcome host barriers remains poorly studied. In this study, ten clinical isolates of C. sakazakii were assessed for their ability to invade and translocate through human microvascular endothelial cells (HBMEC). Their ability to avoid phagocytosis in human macrophages U937 and human brain microglial cells was investigated. Additionally, they were tested for serum sensitivity and the presence of the Cronobacter plasminogen activation gene (cpa) gene, which is reported to confer serum resistance. Our data showed that the clinical C. sakazakii strains invaded and translocated through Caco-2 and HBMEC cell lines and some strains showed significantly higher levels of invasion and translocation. Moreover, C. sakazakii was able to persist and even multiply in phagocytic macrophage and microglial cells. All strains, except one, were able to withstand human serum exposure, the single serum sensitive strain was also the only one which did not encode for the cpa gene. These results demonstrate that C. sakazakii clinical host immune response indicating their capacity to cause diseases such as necrotizing enterocolitis (NEC) and meningitis. Our data showed for the first time the ability of C. sakazakii clinical isolates to survive and multiply within human microglial cells. Additionally, it was shown that C. sakazakii clinical strains have the capacity to translocate through the Caco-2 and HBMEC cell lines paracellularly.
| Item Type: | Journal article | ||||
|---|---|---|---|---|---|
| Publication Title: | Microbial Pathogenesis | ||||
| Creators: | Almajed, F.S. and Forsythe, S.J. | ||||
| Publisher: | Elsevier | ||||
| Date: | 2016 | ||||
| Volume: | 90 | ||||
| ISSN: | 0882-4010 | ||||
| Identifiers: |
|
||||
| Divisions: | Schools > School of Science and Technology | ||||
| Record created by: | Linda Sullivan | ||||
| Date Added: | 23 Feb 2016 14:11 | ||||
| Last Modified: | 09 Jun 2017 13:59 | ||||
| URI: | https://irep.ntu.ac.uk/id/eprint/27013 |
Actions (login required)
 |
Edit View |
Views
Views per month over past year
Downloads
Downloads per month over past year
