The structure of O-polysaccharide isolated from Cronobacter universalis NCTC 9529T

Marszewska, K., Czerwicka, M., Forsythe, S.J. ORCID: 0000-0002-6709-0712, Sałdak, E., Szulta, S., Dziadziuszko, H., Ossowska, K. and Kaczyński, Z., 2014. The structure of O-polysaccharide isolated from Cronobacter universalis NCTC 9529T. Carbohydrate Research, 398, pp. 77-79. ISSN 0008-6215

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Abstract

The O-polysaccharide (OPS) was isolated from Cronobacter universalis NCTC 9529T, a new species in the genus Cronobacter, which was created by the reclassification of the species Enterobacter sakazakii. Purified polysaccharide was analyzed by NMR spectroscopy (1H, COSY, TOCSY, ROESY, HSQC, and HSQC-TOCSY) and chemical methods. The monosaccharide derivatives were analyzed by gas chromatography, and gas chromatography-mass spectrometry. These experiments enabled the type and number of monosaccharides in the repeating unit of OPS, their positions of linkages, and absolute configuration to be determined. Together the chemical analysis established a structure of the OPS of C. universalis NCTC 9529T:
→3)--L-FucpNAc-(1→4)--D-Manp-(1→3)--L-FucpNAc-(1→3)-β-D-GlcpNAc-(1→
[A, B, C, D]
OPS isolated from C. universalis was structurally characterized for the first time.

Item Type: Journal article
Publication Title: Carbohydrate Research
Creators: Marszewska, K., Czerwicka, M., Forsythe, S.J., Sałdak, E., Szulta, S., Dziadziuszko, H., Ossowska, K. and Kaczyński, Z.
Publisher: Elsevier
Date: 2014
Volume: 398
ISSN: 0008-6215
Identifiers:
NumberType
10.1016/j.carres.2014.07.014DOI
Divisions: Schools > School of Science and Technology
Depositing User: Jill Tomkinson
Date Added: 24 Feb 2016 15:49
Last Modified: 09 Jun 2017 13:59
URI: http://irep.ntu.ac.uk/id/eprint/27031

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