Manipulating Rab GTPase activity in wheat to improve gluten quality for breadmaking

Tyler, A.M., Poole, M., Bhandari, D.G., Napier, J.A., Jones, H.D., Lycett, G.W. and Lu, C. ORCID: 0000-0002-0064-4725, 2011. Manipulating Rab GTPase activity in wheat to improve gluten quality for breadmaking. Aspects of Applied Biology, 110, pp. 15-23. ISSN 0265-1491

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Abstract

Sequence information of Rab GTPases in Arabidopsis, rice, Brachypodium and bread wheat was compiled by various means. These included online BLAST and string searches for genes, proteins, ESTs (expressed sequence tags), unigenes and GSSs (genome survey sequences). Sequences of 56 Rab genes from Arabidopsis, 44 from rice, 41 from Brachypodium, three from wheat and 26 wheat unigenes were collected. The sequence information was used to produce dendrograms from amino acid and nucleic acid sequences for comparison of Rab subfamilies in these species. A suitable candidate gene (Ta.54382) was chosen as the target for down-regulation. An RNAi construct was produced, targeting a region of the gene, and used to transform wheat explants.

Item Type: Journal article
Publication Title: Aspects of Applied Biology
Creators: Tyler, A.M., Poole, M., Bhandari, D.G., Napier, J.A., Jones, H.D., Lycett, G.W. and Lu, C.
Publisher: Association of Applied Biologists
Date: 2011
Volume: 110
ISSN: 0265-1491
Divisions: Schools > School of Animal, Rural and Environmental Sciences
Depositing User: Jonathan Gallacher
Date Added: 19 May 2016 12:01
Last Modified: 11 Oct 2017 09:17
URI: http://irep.ntu.ac.uk/id/eprint/27806

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