Circulating hematopoietic stem cells and putative intestinal stem cells in coeliac disease

Piscaglia, A.C., Rutella, S. ORCID: 0000-0003-1970-7375, Laterza, L., Cesario, V., Campanale, M., Cazzato, I.A., Ianiro, G., Barbaro, F., Di Maurizio, L., Bonanno, G., Cenci, T., Cammarota, G., Larocca, L.M. and Gasbarrini, A., 2015. Circulating hematopoietic stem cells and putative intestinal stem cells in coeliac disease. Journal of Translational Medicine, 13, p. 220. ISSN 1479-5876

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Abstract

Background:
The intestinal stem cells (ISC) modulation and the role of circulating hematopoietic stem cells (HSC) in coeliac disease (CD) are poorly understood. Our aim was to investigate the longitudinal modifications in peripheral blood HSC traffic and putative ISC density induced by gluten-free diet (GFD) in CD.
Methods:
Thirty-one CD patients and 7 controls were enrolled. Circulating CD133+ and CD34+ HSC were measured by flow cytometry, at enrolment and after 7 days and 1, 3, 6, 12, and 24 months of GFD. Endoscopy was performed at diagnosis and repeated at 6, 12, and 24 months following GFD. We used the Marsh-Oberhuber score to evaluate the histological severity of duodenal damage; immunohistochemistry was employed to measure the intraepithelial lymphoid infiltrate (IEL, CD3+ lymphoid cells) and the putative ISC compartment (CD133+ and Lgr5+ epithelial cells).
Results:
At enrolment, circulating HSCs were significantly increased in CD patients and they further augmented during the first week of GFD, but progressively decreased afterwards. CD patients presented with villous atrophy, abundant IEL and rare ISC residing at the crypt base. Upon GFD, IEL progressively decreased, while ISC density increased, peaking at 12 months. After 24 months of GFD, all patients were asymptomatic and their duodenal mucosa was macroscopically and histologically normal.
Conclusions:
In active CD patients, the ISC niche is depleted and there is an increased traffic of circulating HSC versus non-coeliac subjects. GFD induces a precocious mobilization of circulating HSC, which is followed by the expansion of the local ISC compartment, leading to mucosal healing and clinical remission.

Item Type: Journal article
Publication Title: Journal of Translational Medicine
Creators: Piscaglia, A.C., Rutella, S., Laterza, L., Cesario, V., Campanale, M., Cazzato, I.A., Ianiro, G., Barbaro, F., Di Maurizio, L., Bonanno, G., Cenci, T., Cammarota, G., Larocca, L.M. and Gasbarrini, A.
Publisher: Biomed Central
Date: July 2015
Volume: 13
ISSN: 1479-5876
Identifiers:
NumberType
10.1186/s12967-015-0591-0DOI
Divisions: Schools > School of Social Sciences
Depositing User: Linda Sullivan
Date Added: 08 Sep 2016 09:09
Last Modified: 09 Jun 2017 14:05
URI: http://irep.ntu.ac.uk/id/eprint/28409

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