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Lilburn, D.M.L., Tatler, A.L., Six, J.S., Lesbats, C., Habgood, A., Porte, J., Hughes-Riley, T. 
ORCID: 0000-0001-8020-430X, Shaw, D.E., Jenkins, G. and Meersmann, T.,
2016.
Investigating lung responses with functional hyperpolarized xenon-129 MRI in an ex vivo rat model of asthma.
Magnetic Resonance in Medicine, 76 (4), pp. 1224-1235.
ISSN 0740-3194
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6479_Hughes-Riley.pdf - Published version Download (6MB) | Preview |
Abstract
Purpose: Asthma is a disease of increasing worldwide importance that calls for new investigative methods. Ex vivo lung tissue is being increasingly used to study functional respiratory parameters independent of confounding systemic considerations but also to reduce animal numbers and associated research costs. In this work, a straightforward laboratory method is advanced to probe dynamic changes in gas inhalation patterns by using an ex vivo small animal ovalbumin (OVA) model of human asthma. Methods: Hyperpolarized (hp)129Xe was actively inhaled by the excised lungs exposed to a constant pressure differential that mimicked negative pleural cavity pressure. The method enabled hp129Xe MRI of airway responsiveness to intravenous methacholine (MCh) and airway challenge reversal through salbutamol. Results: Significant differences were demonstrated between control and OVA challenged animals on global lung hp129Xegas inhalation with P < 0.05 at MCh dosages above 460 µg. Spatial mapping of the regional hp gas distribution revealed an approximately three-fold increase in heterogeneity for the asthma model organs. Conclusion: The experimental results from this proof of concept work suggest that the ex vivo hp noble gas imaging arrangement and the applied image analysis methodology may be useful as an adjunct to current diagnostic techniques.
| Item Type: | Journal article | ||||
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| Publication Title: | Magnetic Resonance in Medicine | ||||
| Creators: | Lilburn, D.M.L., Tatler, A.L., Six, J.S., Lesbats, C., Habgood, A., Porte, J., Hughes-Riley, T., Shaw, D.E., Jenkins, G. and Meersmann, T. | ||||
| Publisher: | John Wiley | ||||
| Date: | October 2016 | ||||
| Volume: | 76 | ||||
| Number: | 4 | ||||
| ISSN: | 0740-3194 | ||||
| Identifiers: |
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| Divisions: | Schools > School of Science and Technology | ||||
| Record created by: | Jonathan Gallacher | ||||
| Date Added: | 24 Oct 2016 14:35 | ||||
| Last Modified: | 12 Sep 2018 10:08 | ||||
| URI: | https://irep.ntu.ac.uk/id/eprint/28931 |
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