Comprehensive glycosylation profiling of IgG and IgG-fusion proteins by top-down MS with multiple fragmentation techniques

Tran, B.Q., Barton, C., Feng, J., Sandjong, A., Yoon, S.H., Awasthi, S., Liang, T., Khan, M.M., Kilgour, D.P.A. ORCID: 0000-0002-3860-7532, Goodlett, D.R. and Goo, Y.A., 2016. Comprehensive glycosylation profiling of IgG and IgG-fusion proteins by top-down MS with multiple fragmentation techniques. Journal of Proteomics, 134, pp. 93-101. ISSN 1874-3919

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Abstract

We employed top- and middle-down analyses with multiple fragmentation techniques including electron transfer dissociation (ETD), electron capture dissociation (ECD), and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) for characterization of a reference monoclonal antibody (mAb) IgG1 and a fusion IgG protein. Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) on an Orbitrap was employed. These experiments provided a comprehensive view on the protein species; especially for different glycosylation level in these two proteins, which showed good agreement with oligosaccharide profiling. Top- and middle-down MS provided additional information regarding glycosylation sites and different combinational protein species that were not available from oligosaccharide mapping or conventional bottom-up analysis. Finally, incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme of Streptococcus pyogene (IdeS) with MALDI-ISD analysis enabled extended sequence coverage of the internal region of protein without pre-fractionation. Biological significance: Oligosaccharide profiling together with top- and middle-down methods enabled: 1) detection of heterogeneous glycosylated protein species and sites in intact IgG1 and fusion proteins with high mass accuracy, 2) estimation of relative abundance levels of protein species in the sample, 3) confirmation of the protein termini structural information, and 4) improved sequence coverage by MALDI-ISD analysis for the internal regions of the proteins without sample pre-fractionation.

Item Type: Journal article
Publication Title: Journal of Proteomics
Creators: Tran, B.Q., Barton, C., Feng, J., Sandjong, A., Yoon, S.H., Awasthi, S., Liang, T., Khan, M.M., Kilgour, D.P.A., Goodlett, D.R. and Goo, Y.A.
Publisher: Elsevier
Date: 2016
Volume: 134
ISSN: 1874-3919
Identifiers:
NumberType
10.1016/j.jprot.2015.10.021DOI
Divisions: Schools > School of Science and Technology
Depositing User: Jonathan Gallacher
Date Added: 23 Nov 2016 10:42
Last Modified: 09 Jun 2017 14:08
URI: http://irep.ntu.ac.uk/id/eprint/29182

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