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Feng, Y., Yang, P., Xie, Y., Wang, X., McNally, A. 
ORCID: 0000-0002-3099-630X and Zong, Z.,
2015.
Escherichia coli of sequence type 3835 carrying blaNDM-1, blaCTX-M-15, blaCMY-42 and blaSHV-12.
Scientific Reports, 5.
ISSN 2045-2322
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Abstract
New Delhi metallo-β-lactamase (NDM) represents a serious challenge for treatment and public health. A carbapenem-resistant Escherichia coli clinical strain WCHEC13-8 was subjected to antimicrobial susceptibility tests, whole genome sequencing and conjugation experiments. It was resistant to imipenem (MIC, >256 μg/ml) and meropenem (MIC, 128 μg/ml) and belonged to ST3835. blaNDM-1 was the only carbapenemase gene detected. Strain WCHEC13-8 also had a plasmid-borne AmpC gene (blaCMY-42) and two extended-spectrum β-lactamase genes (blaCTX-M-15 and blaSHV-12). blaNDM-1 and blaSHV-12 were carried by a 54-kb IncX3 self-transmissible plasmid, which is identical to plasmid pNDM-HF727 from Enterobacter cloacae. blaCMY-42 was carried by a 64-kb IncI1 plasmid and blaCTX-M-15 was located on a 141-kb plasmid with multiple F replicons (replicon type: F36:A4:B1). blaCMY-42 was in a complicated context and the mobilisation of blaCMY-42 was due to the transposition of IS Ecp1 by misidentifying its right-end boundary. Genetic context of blaNDM-1 in strain WCHEC13-8 was closely related to those on IncX3 plasmids in various Enterobacteriaceae species in China. In conclusion, a multidrug-resistant ST3835 E. coli clinical strain carrying blaNDM-1, blaCTX-M-15, blaCMY-42 and blaSHV-12 was identified. IncX3 plasmids may be making a significant contribution to the dissemination of blaNDM among Enterobacteriaceae in China.
| Item Type: | Journal article | ||||
|---|---|---|---|---|---|
| Publication Title: | Scientific Reports | ||||
| Creators: | Feng, Y., Yang, P., Xie, Y., Wang, X., McNally, A. and Zong, Z. | ||||
| Publisher: | Nature Publishing Group | ||||
| Date: | 2015 | ||||
| Volume: | 5 | ||||
| ISSN: | 2045-2322 | ||||
| Identifiers: |
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| Divisions: | Schools > School of Science and Technology | ||||
| Record created by: | EPrints Services | ||||
| Date Added: | 09 Oct 2015 09:47 | ||||
| Last Modified: | 09 Jun 2017 13:11 | ||||
| URI: | https://irep.ntu.ac.uk/id/eprint/2931 |
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