SAUNDERS, B., PAINELLI, V.D.S., OLIVEIRA, L.F.D., SILVA, V.D.E., SILVA, R.P.D., RIANI, L., FRANCHI, M., GONÇALVES, L.D.S., HARRIS, R.C., ROSCHEL, H., ARTIOLI, G.G., SALE, C. and GUALANO, B., 2016. Twenty-four Weeks β-alanine Supplementation on Carnosine Content, Related Genes, and Exercise. Medicine & Science in Sports & Exercise. ISSN 0195-9131
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Introduction: Skeletal muscle carnosine content can be increased through [beta]-alanine supplementation, but the maximum increase achievable with supplementation is unknown. No study has investigated the effects of prolonged supplementation on carnosine-related genes or exercise capacity. Purpose: To investigate the effects of 24-weeks of [beta]-alanine supplementation on muscle carnosine content, gene expression and high-intensity cycling capacity (CCT110%). Methods: Twenty-five active males were supplemented with 6.4 g[middle dot]day-1 of sustained release [beta]-alanine (BA) or placebo (PL) over a 24-week period. Every 4 weeks participants provided a muscle biopsy and performed the CCT110%. Biopsies were analysed for muscle carnosine content and gene expression (CARNS, TauT, ABAT, CNDP2, PHT1, PEPT2 and PAT1). Results: Carnosine content was increased from baseline at every time point in BA (all P<0.0001; Week 4: +11.37+/-7.03 mmol[middle dot]kg-1dm, Week 8: +13.88+/-7.84 mmol[middle dot]kg-1dm, Week 12: +16.95+/-8.54 mmol[middle dot]kg-1dm, Week 16: +17.63+/-8.42 mmol[middle dot]kg-1dm, Week 20: +21.20+/-7.86 mmol[middle dot]kg-1dm, Week 24: +20.15+/-7.63 mmol[middle dot]kg-1dm), but not PL (all P=1.00). Maximal changes were +25.66+/-7.63 mmol[middle dot]kg-1dm (range: +17.13 to +41.32 mmol[middle dot]kg-1dm), and absolute maximal content was 48.03+/-8.97 mmol[middle dot]kg-1dm (range: 31.79 to 63.92 mmol[middle dot]kg-1dm). There was an effect of supplement (P=0.002) on TauT; no further differences in gene expression were shown. Exercise capacity was improved in BA (P=0.05) with possible to almost certain improvements across all weeks. Conclusions: Twenty-four weeks of [beta]-alanine supplementation increased muscle carnosine content and improved high-intensity cycling capacity. Downregulation of TauT suggests it plays an important role in muscle carnosine accumulation with [beta]-alanine supplementation, while the variability in changes in muscle carnosine content between individuals suggests that other determinants other than the availability of [beta]-alanine may also bear a major influence on muscle carnosine content.
|Item Type:||Journal article|
|Publication Title:||Medicine & Science in Sports & Exercise|
|Creators:||Saunders, B., Painelli, V.D.S., Oliveira, L.F.D., Silva, V.D.E., Silva, R.P.D., Riani, L., Franchi, M., Gonçalves, L.D.S., Harris, R.C., Roschel, H., Artioli, G.G., Sale, C. and Gualano, B.|
|Publisher:||Lippincott Williams & Wilkins for the American College of Sports Medicine|
|Divisions:||Schools > School of Science and Technology|
|Depositing User:||Linda Sullivan|
|Date Added:||12 Dec 2016 15:02|
|Last Modified:||12 Dec 2016 15:02|
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