Platelet Lysate-Loaded Photocrosslinkable Hyaluronic Acid Hydrogels for Periodontal Endogenous Regenerative Technology

Babo, P.S., Pires, R.L., Santos, L. ORCID: 0000-0002-1915-6780, Franco, A., Rodrigues, F., Leonor, I., Reis, R.L. and Gomes, M.E., 2016. Platelet Lysate-Loaded Photocrosslinkable Hyaluronic Acid Hydrogels for Periodontal Endogenous Regenerative Technology. ACS Biomaterials Science & Engineering. ISSN 2373-9878

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Abstract

The integrity and function of the periodontium can be compromised by traumatic injuries or periodontitis. Currently available clinical therapies are able to stop the progression of periodontitis and allow the healing of periodontal tissue. However an optimal strategy capable of restoring the anatomy and functionality of the lost periodontal tissue is still to be achieved. Herein is proposed the development of an injectable hydrogel system able to release a growth factors and cells to the periodontal defect. This injectable system is based on a photocrosslinkable hydrogel, prepared from methacrylated Hyaluronic Acid (me-HA) and incorporating Platelet Lysate (PL). The delivery of growth factors and cells in situ is expected to enhance regeneration of the periodontium. Various formulations of me-HA containing increasing PL concentrations were studied for achieving the formation of stable photocrosslinkable hydrogels. The produced hydrogels were subsequently characterized to assess mechanical properties, degradation, protein/growth factor release profile, antimicrobial activity and response towards human Periodontal Ligament fibroblasts (hPDLFs). The results demonstrated that it was possible to obtain stable photocrosslinkable hydrogels incorporating different amounts of PL that can be released in a sustained manner. Furthermore, the incorporation of PL improved (p<0.02) the viscoelastic properties of the hydrogels and enhanced their resilience to the degradation by hyaluronidase (HAase). Additionally, the PL showed to provide antimicrobial properties. Finally, hPDLFs, either seeded or encapsulated into the developed hydrogels, showed enhanced proliferation over time (p<0.05), proportionally to the increasing amounts of PL present in the hydrogel formulations.

Item Type: Journal article
Publication Title: ACS Biomaterials Science & Engineering
Creators: Babo, P.S., Pires, R.L., Santos, L., Franco, A., Rodrigues, F., Leonor, I., Reis, R.L. and Gomes, M.E.
Publisher: American Chemical Society
Date: 20 December 2016
ISSN: 2373-9878
Identifiers:
NumberType
10.1021/acsbiomaterials.6b00508DOI
Divisions: Schools > School of Science and Technology
Depositing User: Jonathan Gallacher
Date Added: 12 Jan 2017 10:54
Last Modified: 09 Jun 2017 14:10
URI: http://irep.ntu.ac.uk/id/eprint/29648

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