Tools
Tools
McDowell, M.A., Marcoux, J., McVicker, G. 
ORCID: 0000-0002-6967-5968, Johnson, S., Fong, Y.H., Stevens, R., Bowman, L.A.H., Degiacomi, M.T., Yan, J., Wise, A., Friede, M.E., Benesch, J.L.P., Deane, J.E., Tang, C.M., Robinson, C.V. and Lea, S.M.,
2016.
Characterisation ofShigella Spa33 andThermotoga FliM/N reveals a new model for C-ring assembly in T3SS.
Molecular Microbiology, 99 (4), pp. 749-766.
ISSN 0950-382X
|
Text
7223_McVicker.pdf - Published version Download (2MB) | Preview |
Abstract
Flagellar type III secretion systems (T3SS) contain an essential cytoplasmic-ring (C-ring) largely composed of two proteins FliM and FliN, whereas an analogous substructure for the closely related non-flagellar (NF) T3SS has not been observed in situ. We show that the spa33 gene encoding the putative NF-T3SS C-ring component in Shigella flexneri is alternatively translated to produce both full-length (Spa33-FL) and a short variant (Spa33-C), with both required for secretion. They associate in a 1:2 complex (Spa33-FL/C2) that further oligomerises into elongated arrays in vitro. The structure of Spa33-C2 and identification of an unexpected intramolecular pseudodimer in Spa33-FL reveal a molecular model for their higher order assembly within NF-T3SS. Spa33-FL and Spa33-C are identified as functional counterparts of a FliM–FliN fusion and free FliN respectively. Furthermore, we show that Thermotoga maritima FliM and FliN form a 1:3 complex structurally equivalent to Spa33-FL/C2, allowing us to propose a unified model for C-ring assembly by NF-T3SS and flagellar-T3SS.
| Item Type: | Journal article | ||||
|---|---|---|---|---|---|
| Publication Title: | Molecular Microbiology | ||||
| Creators: | McDowell, M.A., Marcoux, J., McVicker, G., Johnson, S., Fong, Y.H., Stevens, R., Bowman, L.A.H., Degiacomi, M.T., Yan, J., Wise, A., Friede, M.E., Benesch, J.L.P., Deane, J.E., Tang, C.M., Robinson, C.V. and Lea, S.M. | ||||
| Publisher: | Wiley-Blackwell | ||||
| Date: | February 2016 | ||||
| Volume: | 99 | ||||
| Number: | 4 | ||||
| ISSN: | 0950-382X | ||||
| Identifiers: |
|
||||
| Divisions: | Schools > School of Science and Technology | ||||
| Record created by: | Jonathan Gallacher | ||||
| Date Added: | 12 Jan 2017 12:09 | ||||
| Last Modified: | 09 Jun 2017 14:10 | ||||
| URI: | https://irep.ntu.ac.uk/id/eprint/29656 |
Actions (login required)
 |
Edit View |
Views
Views per month over past year
Downloads
Downloads per month over past year
