Transgenic tomato plants expressing cucumber mosaic virus coat protein and their resistance to CMV

Yang, R., Xu, H., Long, M., Yu, W., Lu, C. ORCID: 0000-0002-0064-4725, Wu, G.C., Pan, N. and Chen, Z., 1995. Transgenic tomato plants expressing cucumber mosaic virus coat protein and their resistance to CMV. Jiangsu Journal of Agricultural Sciences/Jiangsu Nongye Xuebao, 11 (1), pp. 40-44. ISSN 1000-4440

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Abstract

English abstract:
A chimeric gene containing a cloned cDNA of the coat protein (cp) of cucumber mosaic virus (CMV) was transferred into tomato cells, and 42 transgenic tomato plants have been produced by means of a novel leaf disk transformation with the help of a modified Ti plasmid of Agrobacterium tumefaciens. The results of DNA Southern bolt anaylsis confirmed that the CMV-cp gene was stably integrated into the genome of the transgenic plants. CMV coat protein was expressed in the plant cells tested by Western blot and Dot blot. it was shown that the transgenic plants of tomato were protected from infection by CMV. The rate of systematic infection of the virus is reduced or delayed compared with the control plants. The segregation of the R1 progeny of CMV resistance in a 3:1 ratio indicates a single insertion inherited in a Mendelian fashion.

Item Type: Journal article
Alternative Title: 表达黄瓜花叶病毒外壳蛋白的转基因番茄及其对C M V 的抗性
Description: Chinese language
Publication Title: Jiangsu Journal of Agricultural Sciences/Jiangsu Nongye Xuebao
Creators: Yang, R., Xu, H., Long, M., Yu, W., Lu, C., Wu, G.C., Pan, N. and Chen, Z.
Publisher: Jiangsu Academy of Agricultural Sciences/Jiangsu Sheng Nongye Kexueyuan
Date: 6 November 1995
Volume: 11
Number: 1
ISSN: 1000-4440
Divisions: Schools > School of Animal, Rural and Environmental Sciences
Depositing User: Linda Sullivan
Date Added: 31 May 2017 10:10
Last Modified: 09 Jun 2017 14:14
URI: http://irep.ntu.ac.uk/id/eprint/30828

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