Transformation of antisense ACC synthetase related gene and physiological characteristics of transgenic tomato plants

Lu, C. ORCID: 0000-0002-0064-4725, Xu, H., Yu, W., Yang, R., Cao, X., Zhu, Y. and Chen, Z., 1996. Transformation of antisense ACC synthetase related gene and physiological characteristics of transgenic tomato plants. Jiangsu Journal of Agricultural Sciences/Jiangsu Nongye Xuebao, 12 (1), pp. 41-47. ISSN 1000-4440

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Abstract

摘要ACC 合成酶cDNA 被反向插入载体PMON316, 通过三亲交配, 将表达质粒转移到 农杆菌中, 采用叶盘法转化番茄子叶。经长那霉素筛选及分子杂交检测, 表明, 反义ACC 合成酶基 因已在mRNA 水平表达。对转基因番茄果实的成熟生理指标测定结果说明, 转基因番茄果实成熟 受到严重抑制, 乙烯的峰值仅为正常番茄的25%~30%,
PG 活性有所下降, 果实在室温 一「可贮藏 1 个多月, 品质和其他性状与正常番茄相近。

Item Type: Journal article
Alternative Title: Genetic transformation of antisense cDNA of ACC synthetase in tomato and physiological characters regarding storage property of transgenic tomato fruit. 番茄ACC 合成酶反义cDNA 转化及转基因番茄果实贮藏生理 特性研究
Description: Chinese language
Publication Title: Jiangsu Journal of Agricultural Sciences/Jiangsu Nongye Xuebao
Creators: Lu, C., Xu, H., Yu, W., Yang, R., Cao, X., Zhu, Y. and Chen, Z.
Publisher: Jiangsu Academy of Agricultural Sciences/Jiangsu Sheng Nongye Kexueyuan
Date: 1 December 1996
Volume: 12
Number: 1
ISSN: 1000-4440
Divisions: Schools > School of Animal, Rural and Environmental Sciences
Depositing User: Linda Sullivan
Date Added: 05 Jun 2017 13:15
Last Modified: 09 Jun 2017 14:15
URI: http://irep.ntu.ac.uk/id/eprint/30848

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