Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy

Gillies, D, Gamal, W, Downes, A, Reinwald, Y ORCID: 0000-0001-6733-605X, Yanng, Y, El Haj, A and Bagnaninchi, PO, 2017. Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy. In: Larin, KV and Sampson, DD, eds., Optical elastography and tissue biomechanics. Proceedings of SPIE, San Francisco, California, USA, 28-30 January (Vol. 10067). Bellingham, WA: SPIE. ISBN 9781510605756

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There is an unmet need in tissue engineering for non-invasive, label-free monitoring of cell mechanical behaviour in their physiological environment. Here, we describe a novel optical coherence phase microscopy (OCPM) set-up which can map relative cell mechanical behaviour in monolayers and 3D systems non-invasively, and in real-time. 3T3 and MCF-7 cells were investigated, with MCF-7 demonstrating an increased response to hydrostatic stimulus indicating MCF-7 being softer than 3T3, demonstrating the ability to provide qualitative data on cell mechanical behaviour. Quantitative measurements of 6% agarose beads have been taken with commercial Cell Scale Microsquisher® system demonstrating that their mechanical properties are in the same order of magnitude of cells, indicating that this is an appropriate test sample for the novel method desctibed.

Item Type: Chapter in book
Creators: Gillies, D., Gamal, W., Downes, A., Reinwald, Y., Yanng, Y., El Haj, A. and Bagnaninchi, P.O.
Publisher: SPIE
Place of Publication: Bellingham, WA
Date: 21 February 2017
Volume: IV
ISSN: 0277-786X
Rights: Copyright 2017 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Divisions: Schools > School of Science and Technology
Depositing User: Linda Sullivan
Date Added: 26 Jun 2017 14:09
Last Modified: 10 Jul 2017 10:33

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