Tools
Tools
Ali, J., Ali, N., Jamil, S.U.U., Waseem, H., Khan, K. and Pan, G. 
ORCID: 0000-0003-0920-3018,
2017.
Insight into eco-friendly fabrication of silver nanoparticles by Pseudomonas aeruginosa and its potential impacts.
Journal of Environmental Chemical Engineering, 5 (4), pp. 3266-3272.
ISSN 2213-3437
|
Text
8732_Pan.pdf - Post-print Download (1MB) | Preview |
Abstract
Although green synthesis of nanoparticles (NPs) has replaced conventional physicochemical methods owing to eco-friendly and cost effective nature but molecular mechanism is not known completely. Elucidation of the mechanism is needed to enhance the production of control size synthesis and for understanding the biomineralization process. Here we report the facile, extracellular biosynthesis of silver nanoparticles (AgNPs) by Pseudomonas aeruginosa JP1 through nitrate reductase mediated mechanism. AgNO3 was reduced to AgNPs by cell filtrate exposure. UV-visible spectrum of the reaction mixture depicted reduction of ionic silver (Ag+) to atomic silver (Ag0) by a progressive upsurge in surface plasmon resonance (SPR) band range 435-450 nm. X-ray diffraction analysis showed the 2θ values at 38.08°, 44.52°, 64.42° and 77.44° confirming the crystalline nature and mean diameter [6.5-27.88nm (Ave = 13.44 nm)] of AgNPs. Transmission electron microscopy analysis demonstrated the spherical AgNPs with size range 5-45 nm. Stabilizing proteins and rhamnolipids were recognized by Fourier transform infrared spectroscopy. Nitrate reductase was purified and characterized (molecular weight 65 kDa and specific activity = 5.6 U/mg). To probe the plausible mechanism purified enzyme was retreated with AgNO3. Characteristic SPR bands range (435-450 nm) and Particle-induced x-ray emission results also confirmed the synthesis of AgNPs (59679.5 ppm) in solution. These results demonstrated that, nitrate reductase as a principal reducing agent in the mechanistic pathway of AgNPs synthesis, which leads to the understanding of metal transformation and biomineralization processes for controlling the biogeochemical cycles of silver and other heavy metals.
| Item Type: | Journal article | ||||||
|---|---|---|---|---|---|---|---|
| Publication Title: | Journal of Environmental Chemical Engineering | ||||||
| Creators: | Ali, J., Ali, N., Jamil, S.U.U., Waseem, H., Khan, K. and Pan, G. | ||||||
| Publisher: | Elsevier | ||||||
| Date: | August 2017 | ||||||
| Volume: | 5 | ||||||
| Number: | 4 | ||||||
| ISSN: | 2213-3437 | ||||||
| Identifiers: |
|
||||||
| Divisions: | Schools > School of Animal, Rural and Environmental Sciences | ||||||
| Record created by: | Jonathan Gallacher | ||||||
| Date Added: | 03 Jul 2017 15:28 | ||||||
| Last Modified: | 21 Jun 2018 03:00 | ||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/31172 |
Actions (login required)
 |
Edit View |
Views
Views per month over past year
Downloads
Downloads per month over past year
