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Aunin, E., Broadley, D., Ahmed, M.I. 
ORCID: 0000-0002-9051-7681, Mardaryev, A.N. and Botchkareva, N.V.,
2017.
Exploring a role for regulatory miRNAs in wound healing during ageing: involvement of miR-200c in wound repair.
Scientific Reports, 7: 3257.
ISSN 2045-2322
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Abstract
Multiple factors and conditions can lead to impaired wound healing. Chronic non-healing wounds are a common problem among the elderly. To identify microRNAs negatively impacting the wound repair, global miRNA profiling of wounds collected from young and old mice was performed. A subset of miRNAs that exhibited an age-dependent expression pattern during wound closure was identified, including miR-31 and miR-200c. The expression of miR-200 family members was markedly downregulated upon wounding in both young and aged mice, with an exception of acute upregulation of miR-200c at the early phase of wound healing in aged skin. In unwounded aged skin (versus unwounded younger skin), the level of miR-200c was also found elevated in both human and mice. Overexpression of miR-200c in human ex vivo wounds delayed re-epithelialisation and inhibited cell proliferation in the wound epithelium. Modulation of miR-200c expression in both human and mouse keratinocytes in vitro revealed inhibitory effects of miR-200c on migration, but not proliferation. Accelerated wound closure in vitro induced by anti-miR-200c was associated with upregulation of genes controlling cell migration. Thus, our study identified miR-200c as a critical determinant that inhibits cell migration during skin repair after injury and may contribute to age-associated alterations in wound repair.
| Item Type: | Journal article | ||||||||
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| Publication Title: | Scientific Reports | ||||||||
| Creators: | Aunin, E., Broadley, D., Ahmed, M.I., Mardaryev, A.N. and Botchkareva, N.V. | ||||||||
| Publisher: | Nature Publishing Group | ||||||||
| Date: | 2017 | ||||||||
| Volume: | 7 | ||||||||
| ISSN: | 2045-2322 | ||||||||
| Identifiers: |
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| Divisions: | Schools > School of Science and Technology | ||||||||
| Record created by: | Jonathan Gallacher | ||||||||
| Date Added: | 22 Aug 2017 07:29 | ||||||||
| Last Modified: | 26 Oct 2020 15:51 | ||||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/31455 |
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