Exploring a role for regulatory miRNAs in wound healing during ageing: involvement of miR-200c in wound repair

Aunin, E., Broadley, D., Ahmed, M.I. ORCID: 0000-0002-9051-7681, Mardaryev, A.N. and Botchkareva, N.V., 2017. Exploring a role for regulatory miRNAs in wound healing during ageing: involvement of miR-200c in wound repair. Scientific Reports, 7, p. 3257. ISSN 2045-2322

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Abstract

Multiple factors and conditions can lead to impaired wound healing. Chronic non-healing wounds are a common problem among the elderly. To identify microRNAs negatively impacting the wound repair, global miRNA profiling of wounds collected from young and old mice was performed. A subset of miRNAs that exhibited an age-dependent expression pattern during wound closure was identified, including miR-31 and miR-200c. The expression of miR-200 family members was markedly downregulated upon wounding in both young and aged mice, with an exception of acute upregulation of miR-200c at the early phase of wound healing in aged skin. In unwounded aged skin (versus unwounded younger skin), the level of miR-200c was also found elevated in both human and mice. Overexpression of miR-200c in human ex vivo wounds delayed re-epithelialisation and inhibited cell proliferation in the wound epithelium. Modulation of miR-200c expression in both human and mouse keratinocytes in vitro revealed inhibitory effects of miR-200c on migration, but not proliferation. Accelerated wound closure in vitro induced by anti-miR-200c was associated with upregulation of genes controlling cell migration. Thus, our study identified miR-200c as a critical determinant that inhibits cell migration during skin repair after injury and may contribute to age-associated alterations in wound repair.

Item Type: Journal article
Publication Title: Scientific Reports
Creators: Aunin, E., Broadley, D., Ahmed, M.I., Mardaryev, A.N. and Botchkareva, N.V.
Publisher: Nature Publishing Group
Date: 12 June 2017
Volume: 7
ISSN: 2045-2322
Identifiers:
NumberType
10.1038/s41598-017-03331-6DOI
3331Publisher Item Identifier
Divisions: Schools > School of Science and Technology
Depositing User: Jonathan Gallacher
Date Added: 22 Aug 2017 07:29
Last Modified: 22 Aug 2017 07:29
URI: http://irep.ntu.ac.uk/id/eprint/31455

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