MicroRNA-214 controls skin and hair follicle development by modulating the activity of the Wnt pathway

Ahmed, M.I. ORCID: 0000-0002-9051-7681, Alam, M., Emelianov, V.U., Poterlowicz, K., Patel, A., Sharov, A.A., Mardaryev, A.N. and Botchkareva, N.V., 2014. MicroRNA-214 controls skin and hair follicle development by modulating the activity of the Wnt pathway. The Journal of Cell Biology, 207 (4), pp. 549-567. ISSN 0021-9525

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Abstract

Skin development is governed by complex programs of gene activation and silencing, including microRNA-dependent modulation of gene expression. Here, we show that miR-214 regulates skin morphogenesis and hair follicle (HF) cycling by targeting β-catenin, a key component of the Wnt signaling pathway. miR-214 exhibits differential expression patterns in the skin epithelium, and its inducible overexpression in keratinocytes inhibited proliferation, which resulted in formation of fewer HFs with decreased hair bulb size and thinner hair production. The inhibitory effects of miR-214 on HF development and cycling were associated with altered activities of multiple signaling pathways, including decreased expression of key Wnt signaling mediators β-catenin and Lef-1, and were rescued by treatment with pharmacological Wnt activators. Finally, we identify β-catenin as one of the conserved miR-214 targets in keratinocytes. These data provide an important foundation for further analyses of miR-214 as a key regulator of Wnt pathway activity and stem cell functions during normal tissue homeostasis, regeneration, and aging.

Item Type: Journal article
Publication Title: The Journal of Cell Biology
Creators: Ahmed, M.I., Alam, M., Emelianov, V.U., Poterlowicz, K., Patel, A., Sharov, A.A., Mardaryev, A.N. and Botchkareva, N.V.
Publisher: Rockefeller University Press
Date: 24 November 2014
Volume: 207
Number: 4
ISSN: 0021-9525
Identifiers:
NumberType
10.1083/jcb.201404001DOI
Divisions: Schools > School of Science and Technology
Depositing User: Jonathan Gallacher
Date Added: 22 Aug 2017 08:04
Last Modified: 22 Aug 2017 08:04
URI: http://irep.ntu.ac.uk/id/eprint/31457

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