Comparing circadian dynamics in primary derived stem cells from different sources of human adult tissue

Rogers, E.H., Fawcett, S.A., Pekovic-Vaughan, V. and Hunt, J.A. ORCID: 0000-0002-5168-4778, 2017. Comparing circadian dynamics in primary derived stem cells from different sources of human adult tissue. Stem Cells International, 2017. ISSN 1687-966X

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Abstract

Optimising cell/tissue constructs so that they can be successfully accepted and integrated within a host body is essential in modern tissue engineering. To do this, adult stem cells are frequently utilised, but there are many aspects of their environment in vivo that are not completely understood. There is evidence to suggest that circadian rhythms and daily circadian temporal cues have substantial effects on stem cell activation, cell cycle, and differentiation. It was hypothesised that the circadian rhythm in human adult stem cells differs depending on the source of tissue and that different entraining signals exert differential effects depending on the anatomical source. Dexamethasone and rhythmic mechanical stretch were used to synchronise stem cells derived from the bone marrow, tooth dental pulp, and abdominal subcutaneous adipose tissue, and it was experimentally evidenced that these different stem cells differed in their circadian clock properties in response to different synchronisation mechanisms. The more primitive dental pulp-derived stem cells did not respond as well to the chemical synchronisation but showed temporal clock gene oscillations following rhythmic mechanical stretch, suggesting that incorporating temporal circadian information of different human adult stem cells will have profound implications in optimising tissue engineering approaches and stem cell therapies.

Item Type: Journal article
Publication Title: Stem Cells International
Creators: Rogers, E.H., Fawcett, S.A., Pekovic-Vaughan, V. and Hunt, J.A.
Publisher: Hindawi
Date: 23 October 2017
Volume: 2017
ISSN: 1687-966X
Identifiers:
NumberType
10.1155/2017/2057168DOI
2057168Publisher Item Identifier
Divisions: Schools > School of Science and Technology
Depositing User: Linda Sullivan
Date Added: 25 Oct 2017 12:27
Last Modified: 25 Oct 2017 12:27
URI: http://irep.ntu.ac.uk/id/eprint/31875

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