Intracellular pathways involved in bone regeneration triggered by recombinant silk-silica chimeras

Martín-Moldes, Z., Ebrahimi, D., Plowright, R., Dinjaski, N., Perry, C.C. ORCID: 0000-0003-1517-468X, Buehler, M.J. and Kaplan, D.L., 2017. Intracellular pathways involved in bone regeneration triggered by recombinant silk-silica chimeras. Advanced Functional Materials, p. 1702570. ISSN 1616-301X

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Abstract

Biomineralization at the organic-inorganic interface is critical to many biology material functions in vitro and in vivo. Recombinant silk-silica fusion peptides are organic-inorganic hybrid material systems that can be effectively used to study and control biologically-mediated mineralization due to the genetic basis of sequence control. However, to date, the mechanisms by which these functionalized silk-silica proteins trigger the differentiation of human mesenchymal stem cells (hMSCs) to osteoblasts remain unknown. To address this challenge, we analyzed silk-silica surfaces for silica-hMSC receptor binding and activation, and the intracellular pathways involved in the induction of osteogenesis on these bioengineered biomaterials. The induction of gene expression of αVβ3 integrin, all three Mitogen-activated Protein Kinsases (MAPKs) as well as c-Jun, Runt-related Transcription Factor 2 (Runx2) and osteoblast marker genes was demonstrated upon growth of the hMSCs on the silk-silica materials. This induction of key markers of osteogenesis correlated with the content of silica on the materials. Moreover, computational simulations were performed for silk/silica-integrin binding which showed activation of αVβ3 integrin in contact with silica. This integrated computational and experimental approach provides insight into interactions that regulate osteogenesis towards more efficient biomaterial designs.

Item Type: Journal article
Publication Title: Advanced Functional Materials
Creators: Martín-Moldes, Z., Ebrahimi, D., Plowright, R., Dinjaski, N., Perry, C.C., Buehler, M.J. and Kaplan, D.L.
Publisher: WILEY-VCH Verlag
Date: 4 September 2017
ISSN: 1616-301X
Identifiers:
NumberType
10.1002/adfm.201702570DOI
Divisions: Schools > School of Science and Technology
Depositing User: Jill Tomkinson
Date Added: 02 Nov 2017 16:23
Last Modified: 17 Nov 2017 11:58
URI: http://irep.ntu.ac.uk/id/eprint/31947

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