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Martín-Moldes, Z., Ebrahimi, D., Plowright, R., Dinjaski, N., Perry, C.C. 
ORCID: 0000-0003-1517-468X, Buehler, M.J. and Kaplan, D.L.,
2018.
Intracellular pathways involved in bone regeneration triggered by recombinant silk-silica chimeras.
Advanced Functional Materials, 28 (27): 1702570.
ISSN 1616-301X
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Abstract
Biomineralization at the organic-inorganic interface is critical to many biology material functions in vitro and in vivo. Recombinant silk-silica fusion peptides are organic-inorganic hybrid material systems that can be effectively used to study and control biologically-mediated mineralization due to the genetic basis of sequence control. However, to date, the mechanisms by which these functionalized silk-silica proteins trigger the differentiation of human mesenchymal stem cells (hMSCs) to osteoblasts remain unknown. To address this challenge, we analyzed silk-silica surfaces for silica-hMSC receptor binding and activation, and the intracellular pathways involved in the induction of osteogenesis on these bioengineered biomaterials. The induction of gene expression of αVβ3 integrin, all three Mitogen-activated Protein Kinsases (MAPKs) as well as c-Jun, Runt-related Transcription Factor 2 (Runx2) and osteoblast marker genes was demonstrated upon growth of the hMSCs on the silk-silica materials. This induction of key markers of osteogenesis correlated with the content of silica on the materials. Moreover, computational simulations were performed for silk/silica-integrin binding which showed activation of αVβ3 integrin in contact with silica. This integrated computational and experimental approach provides insight into interactions that regulate osteogenesis towards more efficient biomaterial designs.
| Item Type: | Journal article | ||||
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| Publication Title: | Advanced Functional Materials | ||||
| Creators: | Martín-Moldes, Z., Ebrahimi, D., Plowright, R., Dinjaski, N., Perry, C.C., Buehler, M.J. and Kaplan, D.L. | ||||
| Publisher: | WILEY-VCH Verlag | ||||
| Date: | 4 July 2018 | ||||
| Volume: | 28 | ||||
| Number: | 27 | ||||
| ISSN: | 1616-301X | ||||
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| Divisions: | Schools > School of Science and Technology | ||||
| Record created by: | Jill Tomkinson | ||||
| Date Added: | 02 Nov 2017 16:23 | ||||
| Last Modified: | 04 Sep 2018 03:00 | ||||
| URI: | https://irep.ntu.ac.uk/id/eprint/31947 |
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