Proteomics as a tool for the characterisation of nosocomial pathogens

Rajakaruna, L.K., 2010. Proteomics as a tool for the characterisation of nosocomial pathogens. PhD, Nottingham Trent University.


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The aim of the present investigation was to develop and assess the potential of various proteomic approaches for the characterisation of two major nosocomial pathogens, S. aureus and C. difficile and to further investigate the intraspecies diversity of C. difficile using a genotypic approach. The surface-associated proteins of S. aureus and intracellular stable ribosomal proteins of C. difficile were analysed by MALDI-TOF-MS and the resulting spectra interrogated using two databases viz. MMU (Waters®) and SARAMIS™ (AnagnosTec) respectively. A total of 134 clinical S. aureus isolates were tested using the MMU database and the MicrobeLynx™ software. All were successfully identified with minor contamination errors that corroborated with 16S rRNA sequencing. By contrast, C. difficile isolates were only partially identified (to the genus level) using the MMU database and protocol. Changes in the matrix solution and use of the new database (SARAMIS™) resulted in the correct identification of all C. difficile isolates and, detailed ultra-structural studies indicated that intracellular proteins were the new diagnostic biomarkers.

Item Type: Thesis
Creators: Rajakaruna, L.K.
Date: 2010
Rights: This work is the intellectual property of the author, and may be owned by the Department for Bioanalysis and Horizon Technologies (DBHT), Centre For Infections, Health Protection Agency, London and Nottingham Trent University. You may copy up to 5% of this work for private study, or personal, non-commercial research. Any re-use of the information contained within this document should be fully referenced, quoting the author, title, university, degree level and pagination. Queries or requests for any other use, or if a more substantial copy is required, should be directed in the first instance to the author.
Divisions: Schools > School of Science and Technology
Record created by: EPrints Services
Date Added: 09 Oct 2015 09:33
Last Modified: 09 Oct 2015 09:33

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