Binding mechanism of the model charged dye carboxyfluorescein to hyaluronan/polylysine multilayers

Prokopović, V.Z., Vikulina, A.S. ORCID: 0000-0001-9427-2055, Sustr, D., Shchukina, E.M., Shchukin, D.G. and Volodkin, D.V. ORCID: 0000-0001-7474-5329, 2017. Binding mechanism of the model charged dye carboxyfluorescein to hyaluronan/polylysine multilayers. ACS Applied Materials & Interfaces, 9 (44), pp. 38908-38918. ISSN 1944-8244

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Abstract

Biopolymer-based multilayers become more and more attractive due to the vast span of biological application they can be used for, e.g., implant coatings, cell culture supports, scaffolds. Multilayers have demonstrated superior capability to store enormous amounts of small charged molecules, such as drugs, and release them in a controlled manner; however, the binding mechanism for drug loading into the multilayers is still poorly understood. Here we focus on this mechanism using model hyaluronan/polylysine (HA/PLL) multilayers and a model charged dye, carboxyfluorescein (CF). We found that CF reaches a concentration of 13 mM in the multilayers that by far exceeds its solubility in water. The high loading is not related to the aggregation of CF in the multilayers. In the multilayers, CF molecules bind to free amino groups of PLL; however, intermolecular CF–CF interactions also play a role and (i) endow the binding with a cooperative nature and (ii) result in polyadsorption of CF molecules, as proven by fitting of the adsorption isotherm using the BET model. Analysis of CF mobility in the multilayers by fluorescence recovery after photobleaching has revealed that CF diffusion in the multilayers is likely a result of both jumping of CF molecules from one amino group to another and movement, together with a PLL chain being bound to it. We believe that this study may help in the design of tailor-made multilayers that act as advanced drug delivery platform

Item Type: Journal article
Publication Title: ACS Applied Materials & Interfaces
Creators: Prokopović, V.Z., Vikulina, A.S., Sustr, D., Shchukina, E.M., Shchukin, D.G. and Volodkin, D.V.
Publisher: American Chemical Society
Date: 2017
Volume: 9
Number: 44
ISSN: 1944-8244
Identifiers:
NumberType
10.1021/acsami.7b12449DOI
Divisions: Schools > School of Science and Technology
Depositing User: Linda Sullivan
Date Added: 26 Feb 2018 12:23
Last Modified: 30 Jul 2019 14:38
URI: http://irep.ntu.ac.uk/id/eprint/32799

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