HAGE, a novel cancer/testis antigen with strong potential as a target for immunotherapy against cancers

MATHIEU, M., 2007. HAGE, a novel cancer/testis antigen with strong potential as a target for immunotherapy against cancers. PhD, Nottingham Trent University.

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Abstract

Since van der Bruggen et al. (1991) first identified specific human tumour antigens of the MAGE family, numerous potential immunotherapeutic targets have been discovered, often belonging to the so-called cancer/testis (CT) gene family. In a search for novel epitopes from potential tumour target antigens, HAGE, a CT antigen, has been studied. It was first identified in a sarcoma and has since been reported in several carcinomas and leukaemias at the mRNA level only. This study proposed to investigate HAGE as a potential target for immunotherapy in a murine tumour model. HAGE mRNA was found to be expressed in a small proportion of carcinomas, some melanomas and in a strong proportion of chronic myeloid leukaemias as compared to normal tissues, which do not express HAGE with the exception of testis. HAGE protein levels were also confirmed on tissue sections and in cell lines in order to rule out any post-transcriptional modifications. Furthermore, HAGE has been previously described as member of the DEAD-box family of ATP-dependent RNA helicases but very little is known about its actual function. RNA helicases are involved in various steps of RNA metabolism and their over-expression has often been linked with tumorogenesis. Using a combination of silencing and transfection experiments, HAGE was proven to be critical for tumour cell proliferation.

Item Type: Thesis
Creators: Mathieu, M.
Date: 2007
Rights: This work is the intellectual property of the author, and may also be owned by the research sponsor(s) and/or Nottingham Trent University. You may copy up to 5% of this work for private study, or personal, non-commercial research. Any re-use of the information contained within this document should be fully referenced, quoting the author, title, university, degree level and pagination. Queries or requests for any other use, of if a more substantial copy is required, should be directed in the first instance to the author.
Divisions: Schools > School of Science and Technology
Depositing User: EPrints Services
Date Added: 09 Oct 2015 09:33
Last Modified: 09 Oct 2015 09:33
URI: http://irep.ntu.ac.uk/id/eprint/33

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