A “cell-friendly” window for the interaction of cells with hyaluronic acid/poly-L-lysine multilayers

Madaboosi, N., Uhlig, K., Schmidt, S., Vikulina, A.S. ORCID: 0000-0001-9427-2055, Möhwald, H., Duschl, C. and Volodkin, D. ORCID: 0000-0001-7474-5329, 2018. A “cell-friendly” window for the interaction of cells with hyaluronic acid/poly-L-lysine multilayers. Macromolecular Bioscience, 18 (2): 1700319. ISSN 1616-5187

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Abstract

Polyelectrolyte multilayers assembled from hyaluronic acid (HA) and poly-L-lysine (PLL) are most widely studied showing excellent reservoir characteristics to host molecules of diverse nature; however, thick (HA/PLL)n films were often found cell-repellent. By a systematic study of the adhesion and proliferation of various cells as a function of bilayer number ‘n’ a correlation with the mechanical and chemical properties of films is developed. The following cell lines have been studied: mouse 3T3 and L929 fibroblasts, human foreskin primary fibroblasts VH-Fib, human embryonic kidney HEK-293, human bone cell line U-2-OS, Chinese hamster ovary CHO-K and mouse embryonic stem cells. All cells adhere and spread well in a narrow ‘cell-friendly’ window, identified in the range of n=12-15. At n<12, the film is inhomogeneous and at n>15, the film is cell-repellent for all cell lines. Cellular adhesion correlates with the mechanical properties of the films showing that softer films at higher ‘n’ number exhibiting a significant decrease of the Young’s modulus below 100 kPa are weakly adherent to cells. This trend cannot be reversed even by coating a strong cell-adhesive protein fibronectin onto the film. This indicates that mechanical cues plays a major role for cell behaviour, also in respect to biochemical ones.

Item Type: Journal article
Publication Title: Macromolecular Bioscience
Creators: Madaboosi, N., Uhlig, K., Schmidt, S., Vikulina, A.S., Möhwald, H., Duschl, C. and Volodkin, D.
Publisher: Wiley
Date: February 2018
Volume: 18
Number: 2
ISSN: 1616-5187
Identifiers:
NumberType
10.1002/mabi.201700319DOI
Divisions: Schools > School of Science and Technology
Depositing User: Jonathan Gallacher
Date Added: 05 Apr 2018 12:44
Last Modified: 30 Jul 2019 14:34
URI: http://irep.ntu.ac.uk/id/eprint/33190

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