Biomarkers of oxidative stress: methods and measures of oxidative DNA damage (COMET assay) and telomere shortening

Balasubramanyam, M., Adaikalakoteswari, A. ORCID: 0000-0003-2974-3388, Sameermahmood, Z. and Mohan, V., 2009. Biomarkers of oxidative stress: methods and measures of oxidative DNA damage (COMET assay) and telomere shortening. In: R.M. Uppu, S.N. Murthy, W.A. Pryor and N.L. Parinandi, eds., Free Radicals and Antioxidant Protocols. 2nd ed. Methods in molecular biology, 610 . Totowa, NJ: Humana Press, pp. 245-261. ISBN 9781588297105

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Abstract

Oxidative stress is fast becoming the nutritional and medical buzzword for the twenty-first century. The theoretical importance of oxidative stress in diabetes is highlighted by its potential double impact on metabolic dysfunction on one hand and the vascular system on the other hand. The new concept of oxidative stress, being an important trigger in the onset and progression of diabetes and its complications, emphasizes the need for measurement of markers of oxidation to assess the degree of oxidative stress. While we have been routinely measuring biomarkers in our molecular epidemiology projects, here we discuss the utility of two assays, (a) DNA damage assessment by COMET measurement and (b) telomere length measurement. As DNA damage is efficiently repaired by cellular enzymes, its measurement gives a snapshot view of the level of oxidative stress. The protocol allows for measurement of oxidative DNA damage (FPG-sensitive DNA strand breaks). Telomere length measured by Southern blotting technique allows one to estimate the chronic burden of oxidative stress at the molecular level and is now considered as biomarker of biological aging.

Item Type: Chapter in book
Creators: Balasubramanyam, M., Adaikalakoteswari, A., Sameermahmood, Z. and Mohan, V.
Publisher: Humana Press
Place of Publication: Totowa, NJ
Date: 2009
Volume: 610
ISSN: 1064-3745
Identifiers:
NumberType
10.1007/978-1-60327-029-8_15DOI
20013183PubMed ID
Divisions: Schools > School of Science and Technology
Depositing User: Jonathan Gallacher
Date Added: 14 May 2018 15:44
Last Modified: 14 May 2018 15:50
URI: http://irep.ntu.ac.uk/id/eprint/33579

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