High-intensity interval training augments muscle carnosine in the absence of dietary beta-alanine intake

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de Salles Painelli, V., Nemezio, K.M., Jéssica, A., Franchi, M., Andrade, I., Riani, L.A., Saunders, B., Sale, C. ORCID: 0000-0002-5816-4169, Harris, R.C., Gualano, B. and Artioli, G.G., 2018. High-intensity interval training augments muscle carnosine in the absence of dietary beta-alanine intake. Medicine & Science in Sports & Exercise, 50 (11), pp. 2242-2252. ISSN 0195-9131

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Official URL: http://doi.org/10.1249/mss.0000000000001697

Abstract

Purpose: Cross-sectional studies suggest that training can increase muscle carnosine (MCarn), although longitudinal studies have failed to confirm this. A lack of control for dietary β-alanine intake or muscle fibre type shifting may have hampered their conclusions. The purpose of the present study was to investigate the effects of high-intensity interval training (HIIT) on MCarn.

Methods: Twenty vegetarian men were randomly assigned to a control (CON; n=10) or HIIT (n=10) group. HIIT was carried out on a cycle ergometer for 12 weeks, with progressive volume (6-12 series) and intensity (140-170% lactate threshold [LT]). MCarn was quantified in whole-muscle and individual fibres; expression of selected genes (CARNS, CNDP2, ABAT, TauT and PAT1) and muscle buffering capacity in vitro (βmin vitro) were also determined. Exercise tests were performed to evaluate total work done (TWD), VO2max, ventilatory thresholds (VT) and LT.

Results: TWD, VT, LT, VO2max and βmin vitro were improved in the HIIT group (all P<0.05), but not in CON (p>0.05). MCarn (in mmol·kg-1 dry muscle) increased in the HIIT (15.8±5.7 to 20.6±5.3; p=0.012) but not the CON group (14.3±5.3 to 15.0±4.9; p=0.99). In type I fibres, MCarn increased in the HIIT (from 14.4±5.9 to 16.8±7.6; p=0.047) but not the CON group (from 14.0±5.5 to 14.9±5.4; p=0.99). In type IIa fibres, MCarn increased in the HIIT group (from 18.8±6.1 to 20.5±6.4; p=0.067) but not the CON group (from 19.7±4.5 to 18.8±4.4; p=0.37). No changes in gene expression were shown.

Conclusion: In the absence of any dietary intake of β-alanine, HIIT increased MCarn content. The contribution of increased MCarn to the total increase in βmin vitro appears to be small.

Item Type:

Journal article

Alternative Title:

HIIT augments muscle carnosine in the absence of dietary beta-alanine intake [working title]

Publication Title:

Medicine & Science in Sports & Exercise

Creators:

de Salles Painelli, V., Nemezio, K.M., Jéssica, A., Franchi, M., Andrade, I., Riani, L.A., Saunders, B., Sale, C., Harris, R.C., Gualano, B. and Artioli, G.G.

Publisher:

Lippincott Williams & Wilkins

Date:

November 2018

Volume:

Number:

ISSN:

0195-9131

Identifiers: