HIIT augments muscle carnosine in the absence of dietary beta-alanine intake

de Salles Painelli, V., Nemezio, K.M., Jéssica, A., Franchi, M., Andrade, I., Riani, L.A., Saunders, B., Sale, C. ORCID: 0000-0002-5816-4169, Harris, R.C., Gualano, B. and Artioli, G.G., 2018. HIIT augments muscle carnosine in the absence of dietary beta-alanine intake. Medicine & Science in Sports & Exercise. ISSN 0195-9131

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Abstract

Purpose: Cross-sectional studies suggest that training can increase muscle carnosine (MCarn), although longitudinal studies have failed to confirm this. A lack of control for dietary β-alanine intake or muscle fibre type shifting may have hampered their conclusions. The purpose of the present study was to investigate the effects of high-intensity interval training (HIIT) on MCarn.

Methods: Twenty vegetarian men were randomly assigned to a control (CON; n=10) or HIIT (n=10) group. HIIT was carried out on a cycle ergometer for 12 weeks, with progressive volume (6-12 series) and intensity (140-170% lactate threshold [LT]). MCarn was quantified in whole-muscle and individual fibres; expression of selected genes (CARNS, CNDP2, ABAT, TauT and PAT1) and muscle buffering capacity in vitro (βmin vitro) were also determined. Exercise tests were performed to evaluate total work done (TWD), VO2max, ventilatory thresholds (VT) and LT.

Results: TWD, VT, LT, VO2max and βmin vitro were improved in the HIIT group (all P<0.05), but not in CON (p>0.05). MCarn (in mmol·kg-1 dry muscle) increased in the HIIT (15.8±5.7 to 20.6±5.3; p=0.012) but not the CON group (14.3±5.3 to 15.0±4.9; p=0.99). In type I fibres, MCarn increased in the HIIT (from 14.4±5.9 to 16.8±7.6; p=0.047) but not the CON group (from 14.0±5.5 to 14.9±5.4; p=0.99). In type IIa fibres, MCarn increased in the HIIT group (from 18.8±6.1 to 20.5±6.4; p=0.067) but not the CON group (from 19.7±4.5 to 18.8±4.4; p=0.37). No changes in gene expression were shown.

Conclusion: In the absence of any dietary intake of β-alanine, HIIT increased MCarn content. The contribution of increased MCarn to the total increase in βmin vitro appears to be small.

Item Type: Journal article
Publication Title: Medicine & Science in Sports & Exercise
Creators: de Salles Painelli, V., Nemezio, K.M., Jéssica, A., Franchi, M., Andrade, I., Riani, L.A., Saunders, B., Sale, C., Harris, R.C., Gualano, B. and Artioli, G.G.
Publisher: Lippincott Williams & Wilkins
Date: 21 June 2018
ISSN: 0195-9131
Identifiers:
NumberType
10.1249/mss.0000000000001697DOI
29933348PubMed ID
Divisions: Schools > School of Science and Technology
Depositing User: Jonathan Gallacher
Date Added: 16 Jul 2018 09:38
Last Modified: 21 Jun 2019 03:00
URI: http://irep.ntu.ac.uk/id/eprint/34076

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