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Baker, M., Negus, D. 
ORCID: 0000-0001-9047-4565, Raghunathan, D., Radford, P., Moore, C., Clark, G., Diggle, M., Tyson, J., Twycross, J. and Sockett, R.E.,
2017.
Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer.
Scientific Reports, 7 (1): 8329.
ISSN 2045-2322
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Abstract
In worldwide conditions of increasingly antibiotic-resistant hospital infections, it is important to research alternative therapies. Bdellovibrio bacteriovorus bacteria naturally prey on Gram-negative pathogens, including antibiotic-resistant strains and so B. bacteriovorus have been proposed as "living antibiotics" to combat antimicrobially-resistant pathogens. Predator-prey interactions are complex and can be altered by environmental components. To be effective B. bacteriovorus predation needs to work in human body fluids such as serum where predation dynamics may differ to that studied in laboratory media. Here we combine mathematical modelling and lab experimentation to investigate the predation of an important carbapenem-resistant human pathogen, Klebsiella pneumoniae, by B. bacteriovorus in human serum versus buffer. We show experimentally that B. bacteriovorus is able to reduce prey numbers in each environment, on different timescales. Our mathematical model captures the underlying dynamics of the experimentation, including an initial predation-delay at the predator-prey-serum interface. Our research shows differences between predation in buffer and serum and highlights both the potential and limitations of B. bacteriovorus acting therapeutically against K. pneumoniae in serum, informing future research into the medicinal behaviours and dosing of this living antibacterial.
| Item Type: | Journal article | ||||||
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| Publication Title: | Scientific Reports | ||||||
| Creators: | Baker, M., Negus, D., Raghunathan, D., Radford, P., Moore, C., Clark, G., Diggle, M., Tyson, J., Twycross, J. and Sockett, R.E. | ||||||
| Publisher: | Nature Publishing Group | ||||||
| Date: | 21 August 2017 | ||||||
| Volume: | 7 | ||||||
| Number: | 1 | ||||||
| ISSN: | 2045-2322 | ||||||
| Identifiers: |
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| Rights: | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | ||||||
| Divisions: | Schools > School of Social Sciences | ||||||
| Record created by: | Linda Sullivan | ||||||
| Date Added: | 18 Jul 2018 11:09 | ||||||
| Last Modified: | 18 Jul 2018 11:09 | ||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/34101 |
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