Pathoadaptive mutations of Escherichia coli K1 in experimental neonatal systemic infection

Anjum, M., McCarthy, A.J., Negus, D. ORCID: 0000-0001-9047-4565, Martin, P., Pechincha, C., Oswald, E., Stabler, R.A. and Taylor, P.W., 2016. Pathoadaptive mutations of Escherichia coli K1 in experimental neonatal systemic infection. PLoS ONE, 11 (11): e0166793. ISSN 1932-6203

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Abstract

Although Escherichia coli K1 strains are benign commensals in adults, their acquisition at birth by the newborn may result in life-threatening systemic infections, most commonly sepsis and meningitis. Key features of these infections, including stable gastrointestinal (GI) colonization and age-dependent invasion of the bloodstream, can be replicated in the neonatal rat. We previously increased the capacity of a septicemia isolate of E. coli K1 to elicit systemic infection following colonization of the small intestine by serial passage through two-day-old (P2) rat pups. The passaged strain, A192PP (belonging to sequence type 95), induces lethal infection in all pups fed 2–6 x 106 CFU. Here we use whole-genome sequencing to identify mutations responsible for the threefold increase in lethality between the initial clinical isolate and the passaged derivative. Only four single nucleotide polymorphisms (SNPs), in genes (gloB, yjgV, tdcE) or promoters (thrA) involved in metabolic functions, were found: no changes were detected in genes encoding virulence determinants associated with the invasive potential of E. coli K1. The passaged strain differed in carbon source utilization in comparison to the clinical isolate, most notably its inability to metabolize glucose for growth. Deletion of each of the four genes from the E. coli A192PP chromosome altered the proteome, reduced the number of colonizing bacteria in the small intestine and increased the number of P2 survivors. This work indicates that changes in metabolic potential lead to increased colonization of the neonatal GI tract, increasing the potential for translocation across the GI epithelium into the systemic circulation.

Item Type: Journal article
Publication Title: PLoS ONE
Creators: Anjum, M., McCarthy, A.J., Negus, D., Martin, P., Pechincha, C., Oswald, E., Stabler, R.A. and Taylor, P.W.
Publisher: Public Library of Science
Date: 18 November 2016
Volume: 11
Number: 11
ISSN: 1932-6203
Identifiers:
NumberType
10.1371/journal.pone.0166793DOI
Rights: © 2016 McCarthy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Divisions: Schools > School of Science and Technology
Depositing User: Linda Sullivan
Date Added: 20 Jul 2018 08:16
Last Modified: 20 Jul 2018 08:16
URI: http://irep.ntu.ac.uk/id/eprint/34111

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