Klebsiella pneumoniae subsp. pneumoniae–bacteriophage combination from the caecal effluent of a healthy woman

Hoyles, L. ORCID: 0000-0002-6418-342X, Murphy, J., Neve, H., Heller, K.J., Turton, J.F., Mahony, J., Sanderson, J.D., Hudspith, B., Gibson, G.R., McCartney, A.L. and van Sinderen, D., 2015. Klebsiella pneumoniae subsp. pneumoniae–bacteriophage combination from the caecal effluent of a healthy woman. PeerJ, 3, e1061. ISSN 2167-8359

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Abstract

A sample of caecal effluent was obtained from a female patient who had undergone a routine colonoscopic examination. Bacteria were isolated anaerobically from the sample, and screened against the remaining filtered caecal effluent in an attempt to isolate bacteriophages (phages). A lytic phage, named KLPN1, was isolated on a strain identified as Klebsiella pneumoniae subsp. pneumoniae (capsular type K2, rmpA+). This Siphoviridae phage presents a rosette-like tail tip and exhibits depolymerase activity, as demonstrated by the formation of plaque-surrounding haloes that increased in size over the course of incubation. When screened against a panel of clinical isolates of K. pneumoniae subsp. pneumoniae, phage KLPN1 was shown to infect and lyse capsular type K2 strains, though it did not exhibit depolymerase activity on such hosts. The genome of KLPN1 was determined to be 49,037 bp (50.53 %GC) in length, encompassing 73 predicted ORFs, of which 23 represented genes associated with structure, host recognition, packaging, DNA replication and cell lysis. On the basis of sequence analyses, phages KLPN1 (GenBank: KR262148) and 1513 (a member of the family Siphoviridae, GenBank: KP658157) were found to be two new members of the genus “Kp36likevirus.”

Item Type: Journal article
Publication Title: PeerJ
Creators: Hoyles, L., Murphy, J., Neve, H., Heller, K.J., Turton, J.F., Mahony, J., Sanderson, J.D., Hudspith, B., Gibson, G.R., McCartney, A.L. and van Sinderen, D.
Publisher: PeerJ
Date: 28 July 2015
Volume: 3
ISSN: 2167-8359
Identifiers:
NumberType
10.7717/peerj.1061DOI
26246963PubMed ID
Rights: © 2015 Hoyles et al. This is an open access article distributed under the terms of the Open Government License.
Divisions: Schools > School of Science and Technology
Depositing User: Jill Tomkinson
Date Added: 09 Aug 2018 13:51
Last Modified: 09 Aug 2018 13:51
URI: http://irep.ntu.ac.uk/id/eprint/34306

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