Characterization of virus-like particles associated with the human faecal and caecal microbiota

Hoyles, L. ORCID: 0000-0002-6418-342X, McCartney, A.L., Neve, H., Gibson, G.R., Sanderson, J.D., Heller, K.J. and van Sinderen, D., 2014. Characterization of virus-like particles associated with the human faecal and caecal microbiota. Research in Microbiology, 165 (10), pp. 803-812. ISSN 0923-2508

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Abstract

This work represents an investigation into the presence, abundance and diversity of virus-like particles (VLPs) associated with human faecal and caecal samples. Various methodologies for the recovery of VLPs from faeces were tested and optimized, including successful down-stream processing of such samples for the purpose of an in-depth electron microscopic analysis, pulsed-field gel electrophoresis and efficient DNA recovery. The applicability of the developed VLP characterization method beyond the use of faecal samples was then verified using samples obtained from human caecal fluid.

Item Type:	Journal article
Publication Title:	Research in Microbiology
Creators:	Hoyles, L., McCartney, A.L., Neve, H., Gibson, G.R., Sanderson, J.D., Heller, K.J. and van Sinderen, D.
Publisher:	Elsevier Masson SAS on behalf of the Institut Pasteur
Date:	December 2014
Volume:	165
Number:	10
ISSN:	0923-2508
Identifiers:	Number Type 10.1016/j.resmic.2014.10.006 DOI S0923250814002009 Publisher Item Identifier
Divisions:	Schools > School of Science and Technology
Record created by:	Jill Tomkinson
Date Added:	09 Aug 2018 15:26
Last Modified:	09 Aug 2018 15:26
URI:	https://irep.ntu.ac.uk/id/eprint/34309

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