Transposon insertion mapping with PIMMS – Pragmatic Insertional Mutation Mapping System

Blanchard, A.M. ORCID: 0000-0001-6991-7210, Leigh, J.A., Egan, S.A. and Emes, R.D., 2015. Transposon insertion mapping with PIMMS – Pragmatic Insertional Mutation Mapping System. Frontiers in Genetics, 6: 139. ISSN 1664-8021

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Abstract

The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has been developed for simple conditionally essential genome discovery experiments in bacteria. Capable of using raw sequence data files alongside a FASTA sequence of the reference genome and GFF file, PIMMS will generate a tabulated output of each coding sequence with corresponding mapped insertions accompanied with normalized results enabling streamlined analysis. This allows for a quick assay of the genome to identify conditionally essential genes on a standard desktop computer prioritizing results for further investigation.

Item Type: Journal article
Publication Title: Frontiers in Genetics
Creators: Blanchard, A.M., Leigh, J.A., Egan, S.A. and Emes, R.D.
Publisher: Frontiers Research Foundation
Date: 9 April 2015
Volume: 6
ISSN: 1664-8021
Identifiers:
NumberType
10.3389/fgene.2015.00139DOI
Rights: Copyright © 2015 Blanchard, Leigh, Egan and Emes. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Divisions: Schools > School of Animal, Rural and Environmental Sciences
Depositing User: Jonathan Gallacher
Date Added: 13 Aug 2018 15:49
Last Modified: 13 Aug 2018 15:49
URI: http://irep.ntu.ac.uk/id/eprint/34326

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