Importance of the difference in surface pressures of the cell membrane in doxorubicin resistant cells that do not express Pgp and ABCG2

Bellamy, C., Hill, C., Burton, C., Blanchard, A. ORCID: 0000-0001-6991-7210, Shephard, F. and Rauch, C., 2013. Importance of the difference in surface pressures of the cell membrane in doxorubicin resistant cells that do not express Pgp and ABCG2. Cell Biochemistry and Biophysics, 66 (3), pp. 499-512. ISSN 1085-9195

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Abstract

P-glycoprotein (Pgp) represents the archetypal mechanism of drug resistance. But Pgp alone cannot expel drugs. A small but growing body of works has demonstrated that the membrane biophysical properties are central to Pgp-mediated drug resistance. For example, a change in the membrane surface pressure is expected to support drug–Pgp interaction. An interesting aspect from these models is that under specific conditions, the membrane is predicted to take over Pgp concerning the mechanism of drug resistance especially when the surface pressure is high enough, at which point drugs remain physically blocked at the membrane level. However it remains to be determined experimentally whether the membrane itself could, on its own, affect drug entry into cells that have been selected by a low concentration of drug and that do not express transporters. We demonstrate here that in the case of the drug doxorubicin, alteration of the surface pressure of membrane leaflets drive drug resistance.

Item Type: Journal article
Publication Title: Cell Biochemistry and Biophysics
Creators: Bellamy, C., Hill, C., Burton, C., Blanchard, A., Shephard, F. and Rauch, C.
Publisher: Springer
Date: July 2013
Volume: 66
Number: 3
ISSN: 1085-9195
Identifiers:
NumberType
10.1007/s12013-012-9497-0DOI
9497Publisher Item Identifier
Divisions: Schools > School of Animal, Rural and Environmental Sciences
Depositing User: Linda Sullivan
Date Added: 16 Aug 2018 10:42
Last Modified: 07 Sep 2018 07:01
URI: http://irep.ntu.ac.uk/id/eprint/34340

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