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Blanchard, A.M. 
ORCID: 0000-0001-6991-7210, Jolley, K.A., Maiden, M.C.J., Coffey, T.J., Maboni, G., Staley, C.E., Bollard, N.J., Warry, A., Emes, R.D. ORCID: 0000-0001-6855-5481, Davies, P.L. and Tötemeyer, S.,
2018.
The applied development of a tiered multilocus sequence typing (MLST) scheme for Dichelobacter nodosus.
Frontiers in Microbiology, 9: 551.
ISSN 1664-302X
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Abstract
Dichelobacter nodosus (D. nodosus) is the causative pathogen of ovine footrot, a disease that has a significant welfare and financial impact on the global sheep industry. Previous studies into the phylogenetics of D. nodosus have focused on Australia and Scandinavia, meaning the current diversity in the United Kingdom (U.K.) population and its relationship globally, is poorly understood. Numerous epidemiological methods are available for bacterial typing; however, few account for whole genome diversity or provide the opportunity for future application of new computational techniques. Multilocus sequence typing (MLST) measures nucleotide variations within several loci with slow accumulation of variation to enable the designation of allele numbers to determine a sequence type. The usage of whole genome sequence data enables the application of MLST, but also core and whole genome MLST for higher levels of strain discrimination with a negligible increase in experimental cost. An MLST database was developed alongside a seven loci scheme using publically available whole genome data from the sequence read archive. Sequence type designation and strain discrimination was compared to previously published data to ensure reproducibility. Multiple D. nodosus isolates from U.K. farms were directly compared to populations from other countries. The U.K. isolates define new clades within the global population of D. nodosus and predominantly consist of serogroups A, B and H, however serogroups C, D, E, and I were also found. The scheme is publically available at https://pubmlst.org/dnodosus/.
| Item Type: | Journal article | ||||
|---|---|---|---|---|---|
| Publication Title: | Frontiers in Microbiology | ||||
| Creators: | Blanchard, A.M., Jolley, K.A., Maiden, M.C.J., Coffey, T.J., Maboni, G., Staley, C.E., Bollard, N.J., Warry, A., Emes, R.D., Davies, P.L. and Tötemeyer, S. | ||||
| Publisher: | Frontiers Research Foundation | ||||
| Date: | 23 March 2018 | ||||
| Volume: | 9 | ||||
| ISSN: | 1664-302X | ||||
| Identifiers: |
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| Rights: | Copyright © 2018 Blanchard, Jolley, Maiden, Coffey, Maboni, Staley, Bollard, Warry, Emes, Davies and Tötemeyer. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | ||||
| Divisions: | Schools > School of Animal, Rural and Environmental Sciences | ||||
| Record created by: | Jonathan Gallacher | ||||
| Date Added: | 16 Aug 2018 13:56 | ||||
| Last Modified: | 08 Feb 2023 11:57 | ||||
| URI: | https://irep.ntu.ac.uk/id/eprint/34344 |
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