Lipid droplet remodeling and interaction with mitochondria in mouse brown adipose tissue during cold treatment

Yu, J., Zhang, S., Cui, L., Wang, W., Na, H., Zhu, X., Li, L., Xu, G., Yang, F., Christian, M. ORCID: 0000-0002-1616-4179 and Liu, P., 2015. Lipid droplet remodeling and interaction with mitochondria in mouse brown adipose tissue during cold treatment. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1853 (5), pp. 918-928. ISSN 0167-4889

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Abstract

Brown adipose tissue (BAT) maintains animal body temperature by non-shivering thermogenesis, which is through uncoupling protein 1 (UCP1) that uncouples oxidative phosphorylation and utilizes β-oxidation of fatty acids released from triacylglycerol (TAG) in lipid droplets (LDs). Increasing BAT activity and “browning” other tissues such as white adipose tissue (WAT) can enhance the expenditure of excess stored energy, and in turn reduce prevalence of metabolic diseases. Although many studies have characterized the biology of BAT and brown adipocytes, BAT LDs especially their activation induced by cold exposure remain to be explored. We have isolated LDs from mouse interscapular BAT and characterized the full proteome using mass spectrometry. Both morphological and biochemical experiments showed that the LDs could tightly associate with mitochondria. Under cold treatment mouse BAT started expressing LD structure protein PLIN-2/ADRP and increased expression of PLIN1. Both hormone sensitive lipase (HSL) and adipose TAG lipase (ATGL) were increased in LDs. In addition, isolated BAT LDs showed increased levels of the mitochondrial protein UCP1, and prolonged cold exposure could stimulate BAT mitochondrial cristae biogenesis. These changes were in agreement with the data from transcriptional analysis. Our results provide the BAT LD proteome for the first time and show that BAT LDs facilitate heat production by coupling increasing TAG hydrolysis through recruitment of ATGL and HSL to the organelle and expression of another LD resident protein PLIN2/ADRP, as well as by tightly associating with activated mitochondria. These findings will benefit the study of BAT activation and the interaction between LDs and mitochondria.

Item Type: Journal article
Publication Title: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
Creators: Yu, J., Zhang, S., Cui, L., Wang, W., Na, H., Zhu, X., Li, L., Xu, G., Yang, F., Christian, M. and Liu, P.
Publisher: Elsevier
Date: May 2015
Volume: 1853
Number: 5
ISSN: 0167-4889
Identifiers:
NumberType
10.1016/j.bbamcr.2015.01.020DOI
S016748891500035XPublisher Item Identifier
Rights: Copyright © 2015 Elsevier B.V. All rights reserved.
Divisions: Schools > School of Science and Technology
Depositing User: Linda Sullivan
Date Added: 08 Aug 2019 14:21
Last Modified: 08 Aug 2019 15:31
URI: http://irep.ntu.ac.uk/id/eprint/37199

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