Fibroblast growth factor 7 signalling is disrupted in colorectal cancer and is a potential marker of field cancerisation

Patel, A., Tripathi, G., McTernan, P. ORCID: 0000-0001-9023-0261, Gopalakrishnan, K., Ali, O., Spector, E., Williams, N. and Arasaradnam, R.P., 2019. Fibroblast growth factor 7 signalling is disrupted in colorectal cancer and is a potential marker of field cancerisation. Journal of Gastrointestinal Oncology, 10 (3), pp. 429-436. ISSN 2078-6891

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Abstract

Background: Field cancerisation proposes that there are pre-malignant genetic mutations in the macroscopically normal mucosal tissue around colorectal cancer. This study aims to evaluate fibroblast growth factor 7 (FGF7) tissue expression in the mucosal field around colorectal cancer.

Methods: Gene and protein expression of FGF7, its receptor, FGFR2 and its downstream targets; FRS2α, Erk 1/2 and Akt was measured from mucosal samples in 34 control subjects and 17 cancer patients. Serial samples from tumour, adjacent to tumour and at the resection margin were utilised.

Results: FGF7 gene expression was significantly higher in tumour (2.3-fold), adjacent mucosa (3.2-fold) and resection margin (2.8-fold) of cancer patients compared with control subjects (P<0.01 respectively). However, FGFR2 was down regulated (3.5-fold) in the tumour tissue (P<0.001). Protein expression of FRS2α and Akt was significantly lower in tumour tissue compared with the resection margin in cancer patients (P<0.05 respectively). No differences in protein expression of Erk 1/2 were detected.

Conclusions: FGF7 was elevated in the mucosal field of cancer patients supporting its potential as a biomarker of field cancerisation. Changes in FRS2α, Akt and Erk 1/2 expression in the tumour tissue indicate that with malignant transformation, FGF7 loses its ability to regulate cellular differentiation.

Item Type: Journal article
Publication Title: Journal of Gastrointestinal Oncology
Creators: Patel, A., Tripathi, G., McTernan, P., Gopalakrishnan, K., Ali, O., Spector, E., Williams, N. and Arasaradnam, R.P.
Publisher: Pioneer Bioscience Publishing Company
Date: 1 June 2019
Volume: 10
Number: 3
ISSN: 2078-6891
Identifiers:
NumberType
10.21037/jgo.2019.02.11DOI
Divisions: Schools > School of Science and Technology
Depositing User: Jonathan Gallacher
Date Added: 16 Sep 2019 12:39
Last Modified: 16 Sep 2019 12:39
URI: http://irep.ntu.ac.uk/id/eprint/37671

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