The human in vivo biomolecule corona onto PEGylated liposomes: a proof-of-concept clinical study

Hadjidemetriou, M., McAdam, S., Garner, G., Thackeray, C., Knight, D., Smith, D., Al-Ahmady, Z. ORCID: 0000-0001-7681-3735, Mazza, M., Rogan, J., Clamp, A. and Kostarelos, K., 2019. The human in vivo biomolecule corona onto PEGylated liposomes: a proof-of-concept clinical study. Advanced Materials, 31 (4): 1803335. ISSN 0935-9648

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Abstract

The self‐assembled layered adsorption of proteins onto nanoparticle (NP) surfaces, once in contact with biological fluids, is termed the "protein corona" and it is gradually seen as a determinant factor for the overall biological behavior of NPs. Here, the previously unreported in vivo protein corona formed in human systemic circulation is described. The human‐derived protein corona formed onto PEGylated doxorubicin‐encapsulated liposomes (Caelyx) is thoroughly characterized following the recovery of liposomes from the blood circulation of ovarian carcinoma patients. In agreement with previous investigations in mice, the in vivo corona is found to be molecularly richer in comparison to its counterpart ex vivo corona. The intravenously infused liposomes are able to scavenge the blood pool and surface‐capture low‐molecular‐weight, low‐abundance plasma proteins that cannot be detected by conventional plasma proteomic analysis. This study describes the previously elusive or postulated formation of protein corona around nanoparticles in vivo in humans and illustrates that it can potentially be used as a novel tool to analyze the blood circulation proteome.

Item Type: Journal article
Publication Title: Advanced Materials
Creators: Hadjidemetriou, M., McAdam, S., Garner, G., Thackeray, C., Knight, D., Smith, D., Al-Ahmady, Z., Mazza, M., Rogan, J., Clamp, A. and Kostarelos, K.
Publisher: Wiley
Date: 25 January 2019
Volume: 31
Number: 4
ISSN: 0935-9648
Identifiers:
NumberType
10.1002/adma.201803335DOI
1282901Other
Divisions: Schools > School of Science and Technology
Depositing User: Linda Sullivan
Date Added: 04 Feb 2020 11:46
Last Modified: 20 Feb 2020 11:48
URI: http://irep.ntu.ac.uk/id/eprint/39162

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